WORKLIST ENTRIES (1):
GPR3ORPHANR View alignment View Structure GPR3 orphan receptor signature
Type of fingerprint: COMPOUND with 4 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00644 GPRORPHANR; PR00649 GPR6ORPHANR; PR00650 GPR12ORPHANR
INTERPRO; IPR000984
Creation date 10-DEC-1996; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. EIDNE, K.A., ZABAVNIK, J., PETERS, T., YOSHIDA, S., ANDERSON, L.
AND TAYLOR, P.L.
Cloning, sequencing and tissue distribution of a candidate G protein-coupled
receptor from rat pituitary gland.
FEBS LETT. 292 243-248 (1991).
7. SONG, Z.H., MODI, W. AND BONNER, T.I.
Molecular cloning and chromosomal localization of human genes encoding
three closely related G protein-coupled receptors.
GENOMICS 28 347-349 (1995).
8. WATSON, S. AND ARKINSTALL,
Orphan receptors.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.223-230.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Several 7TM receptors have been cloned but their endogenous ligands are
unknown; these have been termed orphan receptors. A new GPCR, GPR12, was
isolated from a rat pituitary library, and is found in discrete regions
of the brain, pituitary and testis, but is absent in other tissues [6-8].
Three human homologues (GPR12, GPR6 and GPR3) have also been isolated [7].
The 3 genes have been localised to human chromosomal regions 13q12, 6q21
and 1p34.3-p36.1 respectively.
GPR3ORPHANR is a 4-element fingerprint that provides a signature for the
GPR3 orphan receptors. The fingerprint was derived from an initial alignment
of 2 sequences: the motifs were drawn from conserved sections within either
loop or N- and C-terminal regions, focusing on those areas of the alignment
that characterise the GPR3 receptors but distinguish them from the rest of
the GPR family - motifs 1 and 2 span the N-terminus; motif 3 spans the first
external loop; and motif 4 resides at the C-terminus. A single iteration on
OWL29.0 was required to reach convergence, no further sequences being
identified beyond the starting set. A single partial match was found,
RATGPCRA, a rat GPR3 fragment lacking motifs 1 and 4.
An update on SPTR37_9f identified a true set of 2 sequences.
SUMMARY INFORMATION
2 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
4| 2 2 2 2
3| 0 0 0 0
2| 0 0 0 0
--+---------------------
| 1 2 3 4
True positives..
GPR3_HUMAN GPR3_MOUSE
PROTEIN TITLES
GPR3_HUMAN PROBABLE G PROTEIN-COUPLED RECEPTOR GPR3 (ACCA ORPHAN RECEPT
GPR3_MOUSE PROBABLE G PROTEIN-COUPLED RECEPTOR GPR3 (GPCR21) - MUS MUSC
SCAN HISTORY
OWL29_0 1 50 NSINGLE
SPTR37_9f 2 3 NSINGLE
INITIAL MOTIF SETS
GPR3ORPHANR1 Length of motif = 18 Motif number = 1
GPR3 motif I - 1
PCODE ST INT
WGAGSPLAWLSAGSGNVN GPR3_HUMAN 3 3
WGAGSSMAWFSAGSGSVN GPR3_MOUSE 3 3
GPR3ORPHANR2 Length of motif = 19 Motif number = 2
GPR3 motif II - 1
PCODE ST INT
NVSSVGPAEGPTGPAAPLP GPR3_HUMAN 20 -1
NVSSVDPVEEPTGPATLLP GPR3_MOUSE 20 -1
GPR3ORPHANR3 Length of motif = 16 Motif number = 3
GPR3 motif III - 1
PCODE ST INT
AAVFCIGSAEMSLVLV GPR3_HUMAN 98 59
AADFCIGSPEMSLMLV GPR3_MOUSE 98 59
GPR3ORPHANR4 Length of motif = 11 Motif number = 4
GPR3 motif IV - 1
PCODE ST INT
AVCCCCSSSKI GPR3_HUMAN 310 196
AICCCCSTSKI GPR3_MOUSE 310 196
FINAL MOTIF SETS
GPR3ORPHANR1 Length of motif = 18 Motif number = 1
GPR3 motif I - 2
PCODE ST INT
WGAGSPLAWLSAGSGNVN GPR3_HUMAN 3 3
WGAGSSMAWFSAGSGSVN GPR3_MOUSE 3 3
GPR3ORPHANR2 Length of motif = 19 Motif number = 2
GPR3 motif II - 2
PCODE ST INT
NVSSVGPAEGPTGPAAPLP GPR3_HUMAN 20 -1
NVSSVDPVEEPTGPATLLP GPR3_MOUSE 20 -1
GPR3ORPHANR3 Length of motif = 16 Motif number = 3
GPR3 motif III - 2
PCODE ST INT
AAVFCIGSAEMSLVLV GPR3_HUMAN 98 59
AADFCIGSPEMSLMLV GPR3_MOUSE 98 59
GPR3ORPHANR4 Length of motif = 11 Motif number = 4
GPR3 motif IV - 2
PCODE ST INT
AVCCCCSSSKI GPR3_HUMAN 310 196
AICCCCSTSKI GPR3_MOUSE 310 196
User query: Display/Full Code "GPR3ORPHANR"