WORKLIST ENTRIES (1): GPR12ORPHANR View alignment View Structure GPR12 orphan receptor signature Type of fingerprint: COMPOUND with 4 elements Links: PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3 PRINTS; PR00251 BACTRLOPSIN PRINTS; PR00644 GPRORPHANR; PR00648 GPR3ORPHANR; PR00649 GPR6ORPHANR INTERPRO; IPR000599 Creation date 09-DEC-1996; UPDATE 07-JUN-1999 1. ATTWOOD, T.K. AND FINDLAY, J.B.C. Fingerprinting G protein-coupled receptors. PROTEIN ENG. 7(2) 195-203 (1994). 2. ATTWOOD, T.K. AND FINDLAY, J.B.C. G protein-coupled receptor fingerprints. 7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993). 3. BIRNBAUMER, L. G proteins in signal transduction. ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990). 4. CASEY, P.J. AND GILMAN, A.G. G protein involvement in receptor-effector coupling. J.BIOL.CHEM. 263(6) 2577-2580 (1988). 5. ATTWOOD, T.K. AND FINDLAY, J.B.C. Design of a discriminating fingerprint for G protein-coupled receptors. PROTEIN ENG. 6(2) 167-176 (1993). 6. EIDNE, K.A., ZABAVNIK, J., PETERS, T., YOSHIDA, S., ANDERSON, L. AND TAYLOR, P.L. Cloning, sequencing and tissue distribution of a candidate G protein-coupled receptor from rat pituitary gland. FEBS LETT. 292 243-248 (1991). 7. WATSON, S. AND ARKINSTALL, Orphan receptors. IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.223-230. G protein-coupled receptors (GPCRs) constitute a vast protein family that encompasses a wide range of functions (including various autocrine, para- crine and endocrine processes). They show considerable diversity at the sequence level, on the basis of which they can be separated into distinct groups. We use the term clan to describe the GPCRs, as they embrace a group of families for which there are indications of evolutionary relationship, but between which there is no statistically significant similarity in sequence [1,2]. The currently known clan members include the rhodopsin-like GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating pheromone receptors, and the metabotropic glutamate receptor family. The rhodopsin-like GPCRs themselves represent a widespread protein family that includes hormone, neurotransmitter and light receptors, all of which transduce extracellular signals through interaction with guanine nucleotide-binding (G) proteins. Although their activating ligands vary widely in structure and character, the amino acid sequences of the receptors are very similar and are believed to adopt a common structural framework comprising 7 transmembrane (TM) helices [3-5]. Several 7TM receptors have been cloned but their endogenous ligands are unknown; these have been termed orphan receptors. GPR12 was isolated from a rat pituitary library and is found in discrete regions of the brain, pituitary and testis, but is absent in other tissues [6,7]. GPR12ORPHANR is a 4-element fingerprint that provides a signature for the GPR12 orphan receptors. The fingerprint was derived from an initial alignment of 4 sequences: the motifs were drawn from conserved sections within either loop or N-terminal regions, focusing on those areas of the alignment that characterise the GPR12 receptors but distinguish them from the rest of the GPR family - motifs 1 and 2 span the N-terminus; motif 3 spans the C-terminus of TM domain 2 and the first external loop; and motif 4 lies in the third external loop. A single iteration on OWL29.0 was required to reach convergence, no further sequences being identified beyond the starting set. An update on SPTR37_9f identified a true set of 3 sequences. SUMMARY INFORMATION 3 codes involving 4 elements 0 codes involving 3 elements 0 codes involving 2 elements COMPOSITE FINGERPRINT INDEX 4| 3 3 3 3 3| 0 0 0 0 2| 0 0 0 0 --+--------------------- | 1 2 3 4 True positives.. GPRC_MOUSE GPRC_RAT GPRC_HUMAN PROTEIN TITLES GPRC_MOUSE PROBABLE G PROTEIN-COUPLED RECEPTOR GPR12 (GPCR01) - MUS MUS GPRC_RAT PROBABLE G PROTEIN-COUPLED RECEPTOR GPR12 (R334) - RATTUS NO GPRC_HUMAN PROBABLE G PROTEIN-COUPLED RECEPTOR GPR12 - HOMO SAPIENS (HU
SCAN HISTORY OWL29_0 1 30 NSINGLE SPTR37_9f 2 4 NSINGLE INITIAL MOTIF SETS GPR12ORPHANR1 Length of motif = 21 Motif number = 1 GPR12 motif I - 1 PCODE ST INT EDPKVNLSGLPRDCIDAGAPE GPRC_MOUSE 3 3 EDPKVNLSGLPRDCIEAGTPE GPRC_RAT 3 3 EDPKVNLSGLPRDCIEAGTPE RNCGPCR 3 3 EDLKVNLSGLPRDYLDAAAAE GPRC_HUMAN 3 3 GPR12ORPHANR2 Length of motif = 17 Motif number = 2 GPR12 motif II - 1 PCODE ST INT ENISAAVPSQGSVAESE GPRC_MOUSE 23 -1 ENISAAVPSQGSVVESE GPRC_RAT 23 -1 ENISAAVPSQGSVVESE RNCGPCR 23 -1 ENISAAVSSRVPAVEPE GPRC_HUMAN 23 -1 GPR12ORPHANR3 Length of motif = 16 Motif number = 3 GPR12 motif III - 1 PCODE ST INT IINFVFAYLLQSEATK GPRC_MOUSE 98 58 IINFVFAYLLQSEATK GPRC_RAT 98 58 IINFVFAYLLQSEATK RNCGPCR 98 58 ITNFVFAYLLQSEATK GPRC_HUMAN 98 58 GPR12ORPHANR4 Length of motif = 11 Motif number = 4 GPR12 motif IV - 1 PCODE ST INT SLIADYTYPSI GPRC_MOUSE 271 157 SLIADYTYPSI GPRC_RAT 271 157 SLIADYTYPSI RNCGPCR 270 156 SLIADYTYPSI GPRC_HUMAN 271 157 FINAL MOTIF SETS GPR12ORPHANR1 Length of motif = 21 Motif number = 1 GPR12 motif I - 2 PCODE ST INT EDPKVNLSGLPRDCIDAGAPE GPRC_MOUSE 3 3 EDPKVNLSGLPRDCIEAGTPE GPRC_RAT 3 3 EDLKVNLSGLPRDYLDAAAAE GPRC_HUMAN 3 3 GPR12ORPHANR2 Length of motif = 17 Motif number = 2 GPR12 motif II - 2 PCODE ST INT ENISAAVPSQGSVAESE GPRC_MOUSE 23 -1 ENISAAVPSQGSVVESE GPRC_RAT 23 -1 ENISAAVSSRVPAVEPE GPRC_HUMAN 23 -1 GPR12ORPHANR3 Length of motif = 16 Motif number = 3 GPR12 motif III - 2 PCODE ST INT IINFVFAYLLQSEATK GPRC_MOUSE 98 58 IINFVFAYLLQSEATK GPRC_RAT 98 58 ITNFVFAYLLQSEATK GPRC_HUMAN 98 58 GPR12ORPHANR4 Length of motif = 11 Motif number = 4 GPR12 motif IV - 2 PCODE ST INT SLIADYTYPSI GPRC_MOUSE 271 157 SLIADYTYPSI GPRC_RAT 271 157 SLIADYTYPSI GPRC_HUMAN 271 157
User query: Display/Full Code "GPR12ORPHANR"