WORKLIST ENTRIES (1):
GPCRSTE2 View alignment Fungal pheromone mating factor STE2 GPCR signature
Type of fingerprint: COMPOUND with 7 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00899 GPCRSTE3; PR00251 BACTRLOPSIN
INTERPRO; IPR000366
Creation date 09-OCT-1993; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. NAKAYAMA, N., MIYAJIMA, A. AND ARAI, K.
Nucleotide sequences of STE2 and STE3, cell type-specific sterile genes
from Saccharomyces cerevisiae.
EMBO J. 4(10) 2643-2648 (1985).
3. BURKHOLDER, A.C. AND HARTWELL, L.H.
The yeast alpha-factor receptor - Structural properties deduced from the
sequence of the STE2 gene.
NUCLEIC ACIDS RES. 13(23) 8463-8475 (1985).
4. MARSH, L. AND HERSKOWITZ, I.
STE2 protein of Saccharomyces kluyveri is a member of the rhodopsin
beta-adrenergic receptor family and is responsible for recognition of
the peptide ligand alpha-factor.
PROC.NATL.ACAD.SCI.U.S.A. 85(11) 3855-3859 (1988).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
Little is known about the structure and function of the mating factor
receptors, STE2 and STE3. It is believed, however, that they are integral
membrane proteins that may be involved in the response to mating factors
on the cell membrane [2-4]. The amino acid sequences of both receptors
contain high proportions of hydrophobic residues grouped into 7 domains,
in a manner reminiscent of the rhodopsins and other receptors believed to
interact with G proteins. However, while a similar 3D framework has been
proposed to account for this, there is no significant sequence similarity
either between STE2 and STE3, or between these and the rhodopsin-type
family: the receptors thus bear their own unique '7TM' signatures.
GPCRSTE2 is a 7-element fingerprint that provides a signature for the
STE2-type family of GPCRs. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs encode the 7 hydrophobic, putative
membrane-spanning regions. A single iteration on OWL21.1 was required to
reach convergence, no further sequences being identified beyond the
starting set.
An update on SPTR37_9f identified a true set of 3 sequences.
SUMMARY INFORMATION
3 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
7| 3 3 3 3 3 3 3
6| 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0
--+------------------------------------
| 1 2 3 4 5 6 7
True positives..
STE2_SACKL STE2_YEAST MAM2_SCHPO
PROTEIN TITLES
STE2_SACKL PHEROMONE ALPHA FACTOR RECEPTOR - SACCHAROMYCES KLUYVERI (YE
STE2_YEAST PHEROMONE ALPHA FACTOR RECEPTOR - SACCHAROMYCES CEREVISIAE (
MAM2_SCHPO PHEROMONE P-FACTOR RECEPTOR - SCHIZOSACCHAROMYCES POMBE (FIS
SCAN HISTORY
OWL23_2 1 100 NSINGLE
OWL28_0 1 100 NSINGLE
SPTR37_9f 2 4 NSINGLE
INITIAL MOTIF SETS
GPCRSTE21 Length of motif = 22 Motif number = 1
STE2 GPCR motif I - 1
PCODE ST INT
GILFGTRIGAAGLAIIVLWMVS STE2_SACKL 51 51
AIMFGVRCGAAALTLIVMWMTS STE2_YEAST 52 52
GPCRSTE22 Length of motif = 25 Motif number = 2
STE2 GPCR motif II - 1
PCODE ST INT
PIFIINQISLFLILLHSSLFLRYLL STE2_SACKL 78 5
PIFIINQVSLFLIILHSALYFKYLL STE2_YEAST 79 5
GPCRSTE23 Length of motif = 24 Motif number = 3
STE2 GPCR motif III - 1
PCODE ST INT
ATNMIQVLLVAAVEISLIFQVRVI STE2_SACKL 129 26
ATNIIQVLLVASIETSLVFQIKVI STE2_YEAST 130 26
GPCRSTE24 Length of motif = 24 Motif number = 4
STE2 GPCR motif IV - 1
PCODE ST INT
GRILTSISAVLGFTTVVMYFITAV STE2_SACKL 162 9
GLMLTSISFTLGIATVTMYFVSAV STE2_YEAST 163 9
GPCRSTE25 Length of motif = 25 Motif number = 5
STE2 GPCR motif V - 1
PCODE ST INT
IASILLSSSVNFMTLLLTVKLILAV STE2_SACKL 206 20
ASTILLASSINFMSFVLVVKLILAI STE2_YEAST 206 19
GPCRSTE26 Length of motif = 24 Motif number = 6
STE2 GPCR motif VI - 1
PCODE ST INT
VLLIMSFQTLIFPSILFILAYALN STE2_SACKL 246 15
ILLIMSCQSLLVPSIIFILAYSLK STE2_YEAST 246 15
GPCRSTE27 Length of motif = 22 Motif number = 7
STE2 GPCR motif VII - 1
PCODE ST INT
TSIATLLVTLSLPLSSMWATSA STE2_SACKL 278 8
TTVATLLAVLSLPLSSMWATAA STE2_YEAST 278 8
FINAL MOTIF SETS
GPCRSTE21 Length of motif = 22 Motif number = 1
STE2 GPCR motif I - 2
PCODE ST INT
GILFGTRIGAAGLAIIVLWMVS STE2_SACKL 51 51
AIMFGVRCGAAALTLIVMWMTS STE2_YEAST 52 52
TLSAQLALGVLTILMVCLLSSS MAM2_SCHPO 51 51
GPCRSTE22 Length of motif = 25 Motif number = 2
STE2 GPCR motif II - 2
PCODE ST INT
PIFIINQISLFLILLHSSLFLRYLL STE2_SACKL 78 5
PIFIINQVSLFLIILHSALYFKYLL STE2_YEAST 79 5
PVFVFNSASIVAMCLRAILNIVTIC MAM2_SCHPO 78 5
GPCRSTE23 Length of motif = 24 Motif number = 3
STE2 GPCR motif III - 2
PCODE ST INT
ATNMIQVLLVAAVEISLIFQVRVI STE2_SACKL 129 26
ATNIIQVLLVASIETSLVFQIKVI STE2_YEAST 130 26
LILLLAPVIIFTAEMSMMIQVRII MAM2_SCHPO 129 26
GPCRSTE24 Length of motif = 24 Motif number = 4
STE2 GPCR motif IV - 2
PCODE ST INT
GRILTSISAVLGFTTVVMYFITAV STE2_SACKL 162 9
GLMLTSISFTLGIATVTMYFVSAV STE2_YEAST 163 9
QRIMTVISACLTVLVLAFWITNMC MAM2_SCHPO 160 7
GPCRSTE25 Length of motif = 25 Motif number = 5
STE2 GPCR motif V - 2
PCODE ST INT
IASILLSSSVNFMTLLLTVKLILAV STE2_SACKL 206 20
ASTILLASSINFMSFVLVVKLILAI STE2_YEAST 206 19
IAKILFAFSIIFHSGVFSYKLFRAI MAM2_SCHPO 209 25
GPCRSTE26 Length of motif = 24 Motif number = 6
STE2 GPCR motif VI - 2
PCODE ST INT
VLLIMSFQTLIFPSILFILAYALN STE2_SACKL 246 15
ILLIMSCQSLLVPSIIFILAYSLK STE2_YEAST 246 15
CILVISCQCLIVPATFTIIDSFIH MAM2_SCHPO 249 15
GPCRSTE27 Length of motif = 22 Motif number = 7
STE2 GPCR motif VII - 2
PCODE ST INT
TSIATLLVTLSLPLSSMWATSA STE2_SACKL 278 8
TTVATLLAVLSLPLSSMWATAA STE2_YEAST 278 8
SSMTQCLLIISLPLSSLWASST MAM2_SCHPO 278 5
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