WORKLIST ENTRIES (1):
GPCRCAMP View alignment cAMP-type GPCR signature
Type of fingerprint: COMPOUND with 7 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00248 GPCRMGR; PR00249 GPCRSECRETIN
PRINTS; PR00250 GPCRSTE2; PR00899 GPCRSTE3; PR00251 BACTRLOPSIN
INTERPRO; IPR000848
Creation date 09-OCT-1993; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. KLEIN, P.S., SUN, T.J., SAXE, C.L., KIMMEL, A.R., JOHNSON, R.L. AND
DEVREOTES, P.N.
A chemoattractant receptor controls development in Dictyostelium discoideum.
SCIENCE 241 1467-1472 (1988).
3. SAXE, C.L., GINSBURG, G.T., LOUIS, J.M., JOHNSON, R., DEVREOTES, P.N.
AND KIMMEL, A.R.
CAR2: A prestalk cAMP receptor required for normal tip formation and late
development of Dictyostelium discoideum.
GENES DEV. 7 262-272 (1993).
4. JOHNSON, R.L., SAXE, C.L., GOLLOP, R., KIMMEL, A.R. AND DEVREOTES, P.N.
Identification and targeted gene disruption of cAR3, a cAMP receptor
subtype expressed during multicellular stages of Dictyostelium development.
GENES DEV. 7 273-282 (1993).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
It has been suggested that the cAMP receptors coordinate aggregation of
individual cells into a multicellular organism, and regulate the expression
of a large number of developmentally-regulated genes [2-4]. The amino acid
sequences of the receptors contain high proportions of hydrophobic residues
grouped into 7 domains, in a manner reminiscent of the rhodopsins and other
receptors believed to interact with G proteins. However, while a similar
3D framework has been proposed to account for this, there is no significant
sequence similarity between these families: the cAMP receptors thus bear
their own unique '7TM' signature.
GPCRCAMP is a 7-element fingerprint that provides a signature for the
cAMP-type family of GPCRs. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs encode the 7 hydrophobic, putative
membrane-spanning regions. A single iteration on OWL21.1 was required to
reach convergence, no further sequences being identified beyond the
starting set.
An update on SPTR37_9f identified a true set of 3 sequences.
SUMMARY INFORMATION
3 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
7| 3 3 3 3 3 3 3
6| 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0
--+------------------------------------
| 1 2 3 4 5 6 7
True positives..
CAR2_DICDI CAR1_DICDI CAR3_DICDI
PROTEIN TITLES
CAR2_DICDI CYCLIC AMP RECEPTOR 2 - DICTYOSTELIUM DISCOIDEUM (SLIME MOLD
CAR1_DICDI CYCLIC AMP RECEPTOR 1 - DICTYOSTELIUM DISCOIDEUM (SLIME MOLD
CAR3_DICDI CYCLIC AMP RECEPTOR 3 - DICTYOSTELIUM DISCOIDEUM (SLIME MOLD
SCAN HISTORY
OWL21_1 1 50 NSINGLE
OWL23_2 1 25 NSINGLE
OWL28_0 1 50 NSINGLE
SPTR37_9f 2 4 NSINGLE
INITIAL MOTIF SETS
GPCRCAMP1 Length of motif = 23 Motif number = 1
cAMP receptor motif I - 1
PCODE ST INT
RTILLIADFSSIIGCSLVLIGFW CAR2_DICDI 10 10
YAVLLIADFTSIIGCTLVLLGFW A46391 23 23
LVLLLFADFSSMLGCMAVLIGFW CAR1_DICDI 13 13
GPCRCAMP2 Length of motif = 21 Motif number = 2
cAMP receptor motif II - 1
PCODE ST INT
IISLFCATSLFKDVISTIITL CAR2_DICDI 44 11
IITFFCSTSLAKDLISTILTL A46391 57 11
VIACFCATSFCKDFPSTILTL CAR1_DICDI 47 11
GPCRCAMP3 Length of motif = 27 Motif number = 3
cAMP receptor motif III - 1
PCODE ST INT
AIVITFGSLALWLWTLMLSFSIYNLIV CAR2_DICDI 81 16
ATVITYGSLACWLWTLCLSFSIYNLIV A46391 93 15
AIVITYGSFACWLWTLCLAISIYMLIV CAR1_DICDI 82 14
GPCRCAMP4 Length of motif = 19 Motif number = 4
cAMP receptor motif IV - 1
PCODE ST INT
FYFCLCYGLPLISTIVMLS CAR2_DICDI 119 11
YYHVFCWVVPFIMSVIMLS A46391 131 11
YYYLLCWGLPLISTIVMLA CAR1_DICDI 120 11
GPCRCAMP5 Length of motif = 21 Motif number = 5
cAMP receptor motif V - 1
PCODE ST INT
FGLFYGPFFFIWGTSAILVGL CAR2_DICDI 159 21
FGLFYGPFLAIWFLAAVLVGL A46391 171 21
FGLFYGPFLFIWAISAVLVGL CAR1_DICDI 160 21
GPCRCAMP6 Length of motif = 23 Motif number = 6
cAMP receptor motif VI - 1
PCODE ST INT
FKLINYIVVFLVCWVFAIVNRIL CAR2_DICDI 204 24
FKLINYIIVFLLCWVFAVINRIV A46391 216 24
FKLINYIIVFLVCWVFAVVNRIV CAR1_DICDI 205 24
GPCRCAMP7 Length of motif = 26 Motif number = 7
cAMP receptor motif VII - 1
PCODE ST INT
FYASITFIYNNPLMWRYFGAKFLLIF CAR2_DICDI 249 22
FYASVTFIYNNPLMWRYLASIILIPF A46391 261 22
FWASVTFIYNNPLMWRYFGAKILTVF CAR1_DICDI 250 22
FINAL MOTIF SETS
GPCRCAMP1 Length of motif = 23 Motif number = 1
cAMP receptor motif I - 2
PCODE ST INT
RTILLIADFSSIIGCSLVLIGFW CAR2_DICDI 11 11
LVLLLFADFSSMLGCMAVLIGFW CAR1_DICDI 14 14
YAVLLIADFTSIIGCTLVLLGFW CAR3_DICDI 24 24
GPCRCAMP2 Length of motif = 21 Motif number = 2
cAMP receptor motif II - 2
PCODE ST INT
IISLFCATSLFKDVISTIITL CAR2_DICDI 45 11
VIACFCATSFCKDFPSTILTL CAR1_DICDI 48 11
IITFFCSTSLAKDLISTILTL CAR3_DICDI 58 11
GPCRCAMP3 Length of motif = 27 Motif number = 3
cAMP receptor motif III - 2
PCODE ST INT
AIVITFGSLALWLWTLMLSFSIYNLIV CAR2_DICDI 82 16
AIVITYGSFACWLWTLCLAISIYMLIV CAR1_DICDI 83 14
ATVITYGSLACWLWTLCLSFSIYNLIV CAR3_DICDI 94 15
GPCRCAMP4 Length of motif = 19 Motif number = 4
cAMP receptor motif IV - 2
PCODE ST INT
FYFCLCYGLPLISTIVMLS CAR2_DICDI 120 11
YYYLLCWGLPLISTIVMLA CAR1_DICDI 121 11
YYHVFCWVVPFIMSVIMLS CAR3_DICDI 132 11
GPCRCAMP5 Length of motif = 21 Motif number = 5
cAMP receptor motif V - 2
PCODE ST INT
FGLFYGPFFFIWGTSAILVGL CAR2_DICDI 160 21
FGLFYGPFLFIWAISAVLVGL CAR1_DICDI 161 21
FGLFYGPFLAIWFLAAVLVGL CAR3_DICDI 172 21
GPCRCAMP6 Length of motif = 23 Motif number = 6
cAMP receptor motif VI - 2
PCODE ST INT
FKLINYIVVFLVCWVFAIVNRIL CAR2_DICDI 205 24
FKLINYIIVFLVCWVFAVVNRIV CAR1_DICDI 206 24
FKLINYIIVFLLCWVFAVINRIV CAR3_DICDI 217 24
GPCRCAMP7 Length of motif = 26 Motif number = 7
cAMP receptor motif VII - 2
PCODE ST INT
FYASITFIYNNPLMWRYFGAKFLLIF CAR2_DICDI 250 22
FWASVTFIYNNPLMWRYFGAKILTVF CAR1_DICDI 251 22
FYASVTFIYNNPLMWRYLASIILIPF CAR3_DICDI 262 22
User query: Display/Full Code "GPCRCAMP"