WORKLIST ENTRIES (1):

GLHYDRLASE56 View alignment View Structure    Glycosyl hydrolase family 56 signature
 Type of fingerprint: COMPOUND with 6  elements
Links:
   PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
   PRINTS; PR00732 GLHYDRLASE4; PR00733 GLHYDRLASE6; PR00734 GLHYDRLASE7
   PRINTS; PR00735 GLHYDRLASE8; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11
   PRINTS; PR00736 GLHYDRLASE15; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20
   PRINTS; PR00739 GLHYDRLASE26; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29
   PRINTS; PR00843 GLHYDRLASE30; PR00742 GLHYDRLASE35; PR00743 GLHYDRLASE36
   PRINTS; PR00744 GLHYDRLASE37; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41
   PRINTS; PR00747 GLHYDRLASE47; PR00844 GLHYDRLASE48; PR00845 GLHYDRLASE52
   PRINTS; PR00849 GLHYDRLASE58; PR00850 GLHYDRLASE59; PR00748 MELIBIASE
   PRINTS; PR00137 LYSOZYME; PR00684 T4LYSOZYME; PR00749 LYSOZYMEG
   PRINTS; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
   PRINTS; PR00847 HYALURONDASE; PR00848 SPERMPH20
   INTERPRO; IPR001968

 Creation date 15-FEB-1998; UPDATE 07-JUN-1999

   1. HENRISSAT, B.
   A classification of glycosyl hydrolases based on amino acid sequence
   similarities.
   BIOCHEM.J. 280 309-316 (1991).

   2. HENRISSAT, B. AND BAIROCH, A.
   New families in the classification of glycosyl hydrolases based on amino
   acid sequence similarities.
   BIOCHEM.J. 293 781-788 (1993).

   3. HENRISSAT, B. AND BAIROCH, A.
   Updating the sequence-based classification of glycosyl hydrolases.
   BIOCHEM.J. 316 695-696 (1996).

   4. EL HASSOUNI, M., HENRISSAT, B., CHIPPAUX, M. AND BARRAS, F.
   Nucleotide sequences of the Arb genes, which control beta-glucosidase
   utilisation in Erwinia chrysanthemi - Comparison with the Escherichia
   coli Bgl operon and evidence for a new beta-glycohydrolase family
   including enzymes from eubacteria, archaebacteria and humans.
   J.BACTERIOL. 174 765-777 (1992).
  
   5. GMACHL, M. AND KREIL, G.
   Bee venom hyaluronidase is homologous to a membrane protein of mammalian
   sperm. 
   PROC.NATL.ACAD.SCI.U.S.A. 90 3569-3573 (1993). 

   6. LATHROP W.F., CARMICHAEL E.P., MYLES D.G., PRIMAKOFF P.
   cDNA cloning reveals the molecular structure of a sperm surface protein, 
   PH-20, involved in sperm-egg adhesion and the wide distribution of its
   gene among mammals. 
   J.CELL BIOL. 111 2939-2949 (1990). 

   O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
   hydrolyse the glycosidic bond between two or more carbohydrates, or between
   a carbohydrate and a non-carbohydrate moiety. A classification system for
   glycosyl hydrolases, based on sequence similarity, has led to the definition
   of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
   glycosid.txt). Family 56 encompasses a group of hyaluronidases (EC 3.2.1.35)
   that includes venom hyaluronidases [5] and mammalian sperm surface proteins
   (PH-20) [6].
  
   The venom of honeybees contains several biologically-active peptides and 
   two enzymes, one of which is a hyaluronidase [5]. The amino acid sequence
   of bee venom hyaluronidase contains 349 amino acids, and includes four
   cysteines and a number of potential glycosylation sites [5]. The sequence
   shows a high degree of similarity to PH-20, a membrane protein of mammalian 
   sperm involved in sperm-egg adhesion, supporting the view that hyaluronidases
   play a role in fertilisation [5]. 
  
   PH-20 is required for sperm adhesion to the egg zona pellucida; it is
   located on both the sperm plasma membrane and acrosomal membrane [6]. The
   amino acid sequence of the mature protein contains 468 amino acids, and
   includes six potential N-linked glycosylation sites and twelve cysteines,
   eight of which are tightly clustered near the C-terminus [6].
  
   GLHYDRLASE56 is a 6-element fingerprint that provides a signature for
   family 56 glycosyl hydrolases. The fingerprint was derived from an initial
   alignment of 13 sequences: the motifs were drawn from conserved regions
   spanning virtually the full alignment length. Two iterations on OWL30.0
   were required to reach convergence, at which point a true set comprising
   18 sequences was identified.
  
   An update on SPTR37_9f identified a true set of 20 sequences.

  SUMMARY INFORMATION
     20 codes involving  6 elements
      0 codes involving  5 elements
      0 codes involving  4 elements
      0 codes involving  3 elements
      0 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    6|  20   20   20   20   20   20  
    5|   0    0    0    0    0    0  
    4|   0    0    0    0    0    0  
    3|   0    0    0    0    0    0  
    2|   0    0    0    0    0    0  
   --+-------------------------------
     |   1    2    3    4    5    6  

True positives..
 O35632         O35631         HYA1_RABIT     HYA1_MACFA     
 HYA1_HUMAN     O15177         Q12891         Q62803         
 Q93013         Q12794         Q29152         HYA1_MOUSE     
 HYA1_CAVPO     O70229         O60540         O43820         
 Q22675         HUGA_DOLMA     HUGA_VESVU     HUGA_APIME     


  PROTEIN TITLES
   O35632           HYALURONIDASE - MUS MUSCULUS (MOUSE).
   O35631           HYALURONIDASE - MUS MUSCULUS (MOUSE).
   HYA1_RABIT       HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN
   HYA1_MACFA       HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN
   HYA1_HUMAN       HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN
   O15177           LYSOSOMAL HYALURONIDASE - HOMO SAPIENS (HUMAN).
   Q12891           LYSOSOMAL HYALURONIDASE (PH-20 HOMOLOG) (LUCA-2) (LUCA2) (PH
   Q62803           PROBABLE HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFAC
   Q93013           HYALURONOGLUCOSAMINIDASE 1 (LUCA-1) (TUMOR SUPPRESSOR LUCA-1
   Q12794           TUMOR SUPPRESSOR (LUCA-1) - HOMO SAPIENS (HUMAN).
   Q29152           PROBABLE HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFAC
   HYA1_MOUSE       HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN
   HYA1_CAVPO       HYALURONIDASE PRECURSOR (EC 3.2.1.35) (SPERM SURFACE PROTEIN
   O70229           HYALURONIDASE 1 (HYALURONOGLUCOSAMINIDASE 1) - MUS MUSCULUS 
   O60540           HYALURONIDASE - HOMO SAPIENS (HUMAN).
   O43820           SIMILAR TO HYALURONOGLUCOSAMINIDASE - HOMO SAPIENS (HUMAN).
   Q22675           T22C8.2 PROTEIN - CAENORHABDITIS ELEGANS.
   HUGA_DOLMA       HYALURONOGLUCOSAMINIDASE (EC 3.2.1.35) (HYALURONIDASE) (ALLE
   HUGA_VESVU       HYALURONOGLUCOSAMINIDASE (EC 3.2.1.35) (HYALURONIDASE) (ALLE
   HUGA_APIME       HYALURONOGLUCOSAMINIDASE PRECURSOR (EC 3.2.1.35) (HYALURONID

SCAN HISTORY OWL30_0 2 40 NSINGLE SPTR37_9f 2 21 NSINGLE INITIAL MOTIF SETS GLHYDRLASE561 Length of motif = 13 Motif number = 1 Glycosyl hydrolase family 56 motif I - 1 PCODE ST INT FLWAWNAPTEFCL HYA1_RABIT 49 49 FVVAWNVPTQECA MMAJ0059 36 36 FLWIWNVPTERCV HYA1_MOUSE 49 49 FVVAWDVPTQDCG AC002455 36 36 FNVYWNVPTFMCH HUGA_APIME 43 43 FLWAWNAPSEFCL HYA1_HUMAN 49 49 FLWAWNAPSEFCL HYA1_MACFA 49 49 FNIYWNVPTFMCH HUGA_DOLMA 8 8 FNIYWNVPTFMCH HUGA_VESVU 8 8 LLWVWNAPTEFCI HYA1_CAVPO 48 48 FTTVWNANTQWCL JC5584 32 32 FTTVWNANTQWCL HSU03056 32 32 TDVVWMVPSWTCK CET22C81 36 36 GLHYDRLASE562 Length of motif = 15 Motif number = 2 Glycosyl hydrolase family 56 motif II - 1 PCODE ST INT VHGRIPQLGPLQQHL HYA1_RABIT 110 48 VHGGVPQNGSLCAHL MMAJ0059 96 47 HYGGIPQRGDYQAHL HYA1_MOUSE 109 47 VHGGVPQNVSLWAHR AC002455 96 47 RNGGVPQLGNLTKHL HUGA_APIME 106 50 VNGGIPQKISLQDHL HYA1_HUMAN 110 48 VHGGIPQKVSLQDHL HYA1_MACFA 110 48 RNGGVPQEGNITIHL HUGA_DOLMA 70 49 RNGGVPQEGNITIHL HUGA_VESVU 70 49 VHGGLPQLMNLQQHL HYA1_CAVPO 109 48 VFGGLPQNASLIAHL JC5584 92 47 VFGGLPQNASLIAHL HSU03056 92 47 KNGGLPQMGDLEAHL CET22C81 97 48 GLHYDRLASE563 Length of motif = 18 Motif number = 3 Glycosyl hydrolase family 56 motif III - 1 PCODE ST INT GLAVIDWEEWLPTWLRNW HYA1_RABIT 141 16 GLAVIDWEEWRPVWVRNW MMAJ0059 128 17 GLAIIDWEEWRPTWLRNW HYA1_MOUSE 140 16 GLAVIDWEDWRPVWVRNW AC002455 128 17 GVGVIDFESWRPIFRQNW HUGA_APIME 138 17 GMAVIDWEEWRPTWARNW HYA1_HUMAN 141 16 GMAVIDWEEWRPTWARNW HYA1_MACFA 141 16 GIGVIDFERWRPIFRQNW HUGA_DOLMA 102 17 GIGVIDFERWRPIFRQNW HUGA_VESVU 102 17 GLAVIDWEEWRPTWTRNW HYA1_CAVPO 140 16 GLAVIDWEAWRPRWAFNW JC5584 124 17 GLAVIDWEAWRPRWAFNW HSU03056 124 17 GIAVIDIEEFRPMWELSW CET22C81 129 17 GLHYDRLASE564 Length of motif = 26 Motif number = 4 Glycosyl hydrolase family 56 motif IV - 1 PCODE ST INT FMEETLKLGRLLRPNHLWGYYLFPDC HYA1_RABIT 199 40 FMLNTLRLRQGSQTQHLWGFYLFPDC MMAJ0059 186 40 FMEGTLHLGKFLRPNQLWGYYLFPDC HYA1_MOUSE 198 40 FMLETLRYVKAVRPRHLWGFYLFPDC AC002455 186 40 FMEETLKAAKRMRPAANWGYYAYPYC HUGA_APIME 196 40 FLVETIKLGKLLRPNHLWGYYLFPDC HYA1_HUMAN 199 40 FMLETIKLGRSLRPNHLWGYYLFPDC HYA1_MACFA 199 40 FMEETLKLAKKTRKQADWGYYGYPYC HUGA_DOLMA 160 40 FMEETLKLAKKTRKQADWGYYGYPYC HUGA_VESVU 160 40 FMLETLKLGKSLRPSSLWGYYLFPDC HYA1_CAVPO 198 40 WMAGTLQLGRALRPRGLWGFYGFPDC JC5584 182 40 WMAGTLQLGGALRPRGLWGFYGFPDC HSU03056 182 40 FFIETLRLGKRLRPNAKWGYYLFPKC CET22C81 187 40 GLHYDRLASE565 Length of motif = 15 Motif number = 5 Glycosyl hydrolase family 56 motif V - 1 PCODE ST INT NDDLSWLWKESTALF HYA1_RABIT 247 22 NDQLAWLWAESTALF MMAJ0059 235 23 NDNLKWLWKASTGLY HYA1_MOUSE 245 21 NDQLAWLWAESTALF AC002455 235 23 NDKMSWLFESEDVLL HUGA_APIME 241 19 NDDLSWLWNESTALY HYA1_HUMAN 246 21 NDDLSWLWNESTALY HYA1_MACFA 246 21 NDKMSWLFNNQNVLL HUGA_DOLMA 205 19 NDKMSWLFNNQNVLL HUGA_VESVU 205 19 NNDLQWLWNDSTALY HYA1_CAVPO 245 21 NDQLGWLWGQSRALY JC5584 229 21 NDQLGWLWGQSRALY HSU03056 229 21 NDNLHWLWGESTALF CET22C81 232 19 GLHYDRLASE566 Length of motif = 14 Motif number = 6 Glycosyl hydrolase family 56 motif VI - 1 PCODE ST INT CLHLDNYMKTILNP HYA1_RABIT 355 93 CQYLKNYLTQLLVP MMAJ0059 339 89 CPILHKYMQTTLNP HYA1_MOUSE 351 91 CQYLKDYLTRLLVP AC002455 340 90 CLQFREYLNNELGP HUGA_APIME 345 89 CLLLDNYMETILNP HYA1_HUMAN 351 90 CLLLDTYMETILNP HYA1_MACFA 351 90 CKRLREYLLTVLGP HUGA_DOLMA 308 88 CKRLQDYLLTVLGP HUGA_VESVU 308 88 CIGLENYMKGTLLP HYA1_CAVPO 351 91 CQAIKEYMDTTLGP JC5584 333 89 CQAIKEYMDTTLGP HSU03056 333 89 CGSLQTYVDNTLGP CET22C81 337 90 FINAL MOTIF SETS GLHYDRLASE561 Length of motif = 13 Motif number = 1 Glycosyl hydrolase family 56 motif I - 2 PCODE ST INT FVVAWNVPTQECA O35631 36 36 FVVAWNVPTQECA O35632 36 36 FLWAWNAPTEFCL HYA1_RABIT 49 49 FLWAWNAPSEFCL HYA1_MACFA 49 49 FLWAWNAPSEFCL HYA1_HUMAN 49 49 FVVAWDVPTQDCG O15177 36 36 FVVAWDVPTQDCG Q12891 36 36 FVWVWNVPTEACV Q62803 49 49 FTTVWNANTQWCL Q93013 32 32 FTTVWNANTQWCL Q12794 32 32 FLWGWNAPTELCA Q29152 49 49 FLWIWNVPTERCV HYA1_MOUSE 49 49 LLWVWNAPTEFCI HYA1_CAVPO 48 48 FITVWNGDTHWCL O70229 60 60 FSVLWNVPSAHCE O43820 31 31 FSVLWNVPSAHCE O60540 31 31 TDVVWMVPSWTCK Q22675 36 36 FNIYWNVPTFMCH HUGA_DOLMA 8 8 FNIYWNVPTFMCH HUGA_VESVU 8 8 FNVYWNVPTFMCH HUGA_APIME 43 43 GLHYDRLASE562 Length of motif = 15 Motif number = 2 Glycosyl hydrolase family 56 motif II - 2 PCODE ST INT VHGGVPQNGSLCAHL O35631 96 47 VHGGVPQNGSLCAHL O35632 96 47 VHGRIPQLGPLQQHL HYA1_RABIT 110 48 VHGGIPQKVSLQDHL HYA1_MACFA 110 48 VNGGIPQKISLQDHL HYA1_HUMAN 110 48 VHGGVPQNVSLWAHR O15177 96 47 VHGGVPQNVSLWAHR Q12891 96 47 HHGGIPQKGDLTTHL Q62803 109 47 VFGGLPQNASLIAHL Q93013 92 47 VFGGLPQNASLIAHL Q12794 92 47 VNGGIPQLGSLKKHL Q29152 110 48 HYGGIPQRGDYQAHL HYA1_MOUSE 109 47 VHGGLPQLMNLQQHL HYA1_CAVPO 109 48 VFGGLPQNASLVTHL O70229 120 47 HNGGIPQALPLDRHL O43820 91 47 HNGGIPQALPLDRHL O60540 91 47 KNGGLPQMGDLEAHL Q22675 97 48 RNGGVPQEGNITIHL HUGA_DOLMA 70 49 RNGGVPQEGNITIHL HUGA_VESVU 70 49 RNGGVPQLGNLTKHL HUGA_APIME 106 50 GLHYDRLASE563 Length of motif = 18 Motif number = 3 Glycosyl hydrolase family 56 motif III - 2 PCODE ST INT GLAVIDWEEWRPVWVRNW O35631 128 17 GLAVIDWEEWRPVWVRNW O35632 128 17 GLAVIDWEEWLPTWLRNW HYA1_RABIT 141 16 GMAVIDWEEWRPTWARNW HYA1_MACFA 141 16 GMAVIDWEEWRPTWARNW HYA1_HUMAN 141 16 GLAVIDWEDWRPVWVRNW O15177 128 17 GLAVIDWEDWRPVWVRNW Q12891 128 17 GLAIIDWEEWRPTWMRNW Q62803 140 16 GLAVIDWEAWRPRWAFNW Q93013 124 17 GLAVIDWEAWRPRWAFNW Q12794 124 17 GLAVIDWDSWRPNWARNW Q29152 141 16 GLAIIDWEEWRPTWLRNW HYA1_MOUSE 140 16 GLAVIDWEEWRPTWTRNW HYA1_CAVPO 140 16 GLAVIDWEAWRPRWAFNW O70229 152 17 GPAVLDWEEWCPLWAGNW O43820 122 16 GPAVLDWEEWCPLWAGNW O60540 122 16 GIAVIDIEEFRPMWELSW Q22675 129 17 GIGVIDFERWRPIFRQNW HUGA_DOLMA 102 17 GIGVIDFERWRPIFRQNW HUGA_VESVU 102 17 GVGVIDFESWRPIFRQNW HUGA_APIME 138 17 GLHYDRLASE564 Length of motif = 26 Motif number = 4 Glycosyl hydrolase family 56 motif IV - 2 PCODE ST INT FMLNTLRLRQGSQTQHLWGFYLFPDC O35631 186 40 FMLNTLRLRQGSQTQHLWGFYLFPDC O35632 186 40 FMEETLKLGRLLRPNHLWGYYLFPDC HYA1_RABIT 199 40 FMLETIKLGRSLRPNHLWGYYLFPDC HYA1_MACFA 199 40 FLVETIKLGKLLRPNHLWGYYLFPDC HYA1_HUMAN 199 40 FMLETLRYVKAVRPRHLWGFYLFPDC O15177 186 40 FMLETLRYVKAVRPRHLWGFYLFPDC Q12891 186 40 FMEGTLKLGKHIRPKHLWGFYLFPDC Q62803 198 40 WMAGTLQLGRALRPRGLWGFYGFPDC Q93013 182 40 WMAGTLQLGGALRPRGLWGFYGFPDC Q12794 182 40 FMQETLKLGKFLRPNYLWGFYLYPDC Q29152 199 40 FMEGTLHLGKFLRPNQLWGYYLFPDC HYA1_MOUSE 198 40 FMLETLKLGKSLRPSSLWGYYLFPDC HYA1_CAVPO 198 40 WMAGTLQLGQVLRPRGLWGYYGFPDC O70229 210 40 LMEDTLRVAQALRPHGLWGFYHYPAC O43820 180 40 LMEDTLRVAQALRPHGLWGFYHYPAC O60540 180 40 FFIETLRLGKRLRPNAKWGYYLFPKC Q22675 187 40 FMEETLKLAKKTRKQADWGYYGYPYC HUGA_DOLMA 160 40 FMEETLKLAKKTRKQADWGYYGYPYC HUGA_VESVU 160 40 FMEETLKAAKRMRPAANWGYYAYPYC HUGA_APIME 196 40 GLHYDRLASE565 Length of motif = 15 Motif number = 5 Glycosyl hydrolase family 56 motif V - 2 PCODE ST INT NDQLAWLWAESTALF O35631 235 23 NDQLAWLWAESTALF O35632 235 23 NDDLSWLWKESTALF HYA1_RABIT 247 22 NDDLSWLWNESTALY HYA1_MACFA 246 21 NDDLSWLWNESTALY HYA1_HUMAN 246 21 NDQLAWLWAESTALF O15177 235 23 NDQLAWLWAESTALF Q12891 235 23 NDDLDWLWKESTGLY Q62803 245 21 NDQLGWLWGQSRALY Q93013 229 21 NDQLGWLWGQSRALY Q12794 229 21 NDEIDWLWKESTALF Q29152 246 21 NDNLKWLWKASTGLY HYA1_MOUSE 245 21 NNDLQWLWNDSTALY HYA1_CAVPO 245 21 NDQLGWLWNQSYALY O70229 257 21 NTQLHWLWAASSALF O43820 228 22 NTQLHWLWAASSALF O60540 228 22 NDNLHWLWGESTALF Q22675 232 19 NDKMSWLFNNQNVLL HUGA_DOLMA 205 19 NDKMSWLFNNQNVLL HUGA_VESVU 205 19 NDKMSWLFESEDVLL HUGA_APIME 241 19 GLHYDRLASE566 Length of motif = 14 Motif number = 6 Glycosyl hydrolase family 56 motif VI - 2 PCODE ST INT CQYLKNYLTQLLVP O35631 339 89 CQYLKNYLTQLLVP O35632 340 90 CLHLDNYMKTILNP HYA1_RABIT 355 93 CLLLDTYMETILNP HYA1_MACFA 351 90 CLLLDNYMETILNP HYA1_HUMAN 351 90 CQYLKDYLTRLLVP O15177 339 89 CQYLKDYLTRLLVP Q12891 340 90 CPILRQYMKTTLNP Q62803 351 91 CQAIKEYMDTTLGP Q93013 333 89 CQAIKEYMDTTLGP Q12794 333 89 CTELDTYIKNKLNP Q29152 352 91 CPILHKYMQTTLNP HYA1_MOUSE 351 91 CIGLENYMKGTLLP HYA1_CAVPO 351 91 CQAIKAYMDSTLGP O70229 361 89 CWHLHDYLVDTLGP O43820 331 88 CWHLHDYLVDTLGP O60540 331 88 CGSLQTYVDNTLGP Q22675 337 90 CKRLREYLLTVLGP HUGA_DOLMA 308 88 CKRLQDYLLTVLGP HUGA_VESVU 308 88 CLQFREYLNNELGP HUGA_APIME 345 89

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