WORKLIST ENTRIES (1):
GLHYDRLASE36 View alignment Glycosyl hydrolase family 36 signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
PRINTS; PR00732 GLHYDRLASE4; PR00733 GLHYDRLASE6; PR00734 GLHYDRLASE7
PRINTS; PR00735 GLHYDRLASE8; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11
PRINTS; PR00736 GLHYDRLASE15; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20
PRINTS; PR00739 GLHYDRLASE26; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29
PRINTS; PR00843 GLHYDRLASE30; PR00742 GLHYDRLASE35; PR00744 GLHYDRLASE37
PRINTS; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41; PR00747 GLHYDRLASE47
PRINTS; PR00844 GLHYDRLASE48; PR00845 GLHYDRLASE52; PR00846 GLHYDRLASE56
PRINTS; PR00849 GLHYDRLASE58; PR00850 GLHYDRLASE59; PR00748 MELIBIASE
PRINTS; PR00137 LYSOZYME; PR00684 T4LYSOZYME; PR00749 LYSOZYMEG
PRINTS; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
INTERPRO; IPR002252
PROSITE; PS00512 ALPHA_GALACTOSIDASE
Creation date 11-JUN-1997; UPDATE 07-JUN-1999
1. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
2. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
3. DAVIES, G. AND HENRISSAT, B.
Structures and mechanisms of glycosyl hydrolases.
STRUCTURE 3 853-859 (1995).
4. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
5. TURAKAINEN, H., KRISTO, P. AND KORHOLA, M.
Consideration of the evolution of the Saccharomyces cerevisiae MEL gene
family on the basis of the nucleotide sequences of the genes and their
flanking regions.
YEAST 10(12) 1559-1568 (1994).
6. ASLANIDIS C., SCHMID K., SCHMITT R.
Nucleotide sequences and operon structure of plasmid-borne genes mediating
uptake and utilization of raffinose in Escherichia coli.
J.BACTERIOL. 171 6753-6763 (1989).
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). Family 36 encompasses alpha-galactosidases.
Alpha-galactosidase (melibiase) catalyses the hydrolysis of melibiose into
galactose and glucose [5]. In man, deficiency in the enzyme results in
Fabry's disease (X-linked sphingolipidosis). An E.coli plasmid-encoded
alpha-galactosidase (rafA) [6] contains a region of about 50 amino acids
that is similar to a domain of the eukaryotic alpha-galactosidases.
GLHYDRLASE36 is an 8-element fingerprint that provides a signature for
family 36 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved regions
spanning the central portion of the alignment - motif 4 includes the
region encoded by PROSITE pattern ALPHA_GALACTOSIDASE (PS00512), which
contains two conserved Asp residues that may be involved in the catalytic
mechanism. Two iterations on OWL29.3 were required to reach convergence, at
which point a true set comprising 5 sequences was identified. A single
partial match was found, TEMELA, an alpha-galactosidase that matches motifs
3 and 6.
An update on SPTR37_9f identified a true set of 5 sequences.
SUMMARY INFORMATION
5 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 5 5 5 5 5 5 5 5
7| 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
AGA1_PEDPE AGAL_STRMU RAFA_ECOLI Q92457
AGA2_PEDPE
PROTEIN TITLES
AGA1_PEDPE ALPHA-GALACTOSIDASE 1 (EC 3.2.1.22) (MELIBIASE) - PEDIOCOCCU
AGAL_STRMU ALPHA-GALACTOSIDASE (EC 3.2.1.22) (MELIBIASE) - STREPTOCOCCU
RAFA_ECOLI ALPHA-GALACTOSIDASE (EC 3.2.1.22) (MELIBIASE) - ESCHERICHIA
Q92457 ALPHA-GALACTOSIDASE PRECURSOR (EC 3.2.1.22) (MELIBIASE) - TR
AGA2_PEDPE ALPHA-GALACTOSIDASE 2 (EC 3.2.1.22) (MELIBIASE) - PEDIOCOCCU
SCAN HISTORY
OWL29_3 2 50 NSINGLE
SPTR37_9f 2 6 NSINGLE
INITIAL MOTIF SETS
GLHYDRLASE361 Length of motif = 16 Motif number = 1
Glycosyl hydrolase family 36 motif I - 1
PCODE ST INT
EFSGDAYGFNLVYSGN AGA1_PEDPE 243 243
EFYGEALALQLIYSGN AGAL_STRMU 235 235
EQQGEVWAVHLGWSGN RAFA_ECOLI 205 205
ELAGTVIGCALAWSGN AGA2_PEDPE 239 239
GLHYDRLASE362 Length of motif = 21 Motif number = 2
Glycosyl hydrolase family 36 motif II - 1
PCODE ST INT
TPEVLMVYTNKGLNAMSQAYH AGA1_PEDPE 294 35
TPVALITYTDKGLTDLTQESH AGAL_STRMU 286 35
TPWLYACHSADGLNGMSQQYH RAFA_ECOLI 256 35
TPEAVLTWTNTGFNGMSQVFH AGA2_PEDPE 290 35
GLHYDRLASE363 Length of motif = 15 Motif number = 3
Glycosyl hydrolase family 36 motif III - 1
PCODE ST INT
PIVVNNWEATFFDFN AGA1_PEDPE 332 17
PILINNWEATYFDFN AGAL_STRMU 324 17
PVHLNTWEGIYFNHN RAFA_ECOLI 293 16
PSVLNTWETLTFAVS AGA2_PEDPE 324 13
GLHYDRLASE364 Length of motif = 16 Motif number = 4
Glycosyl hydrolase family 36 motif IV - 1
PCODE ST INT
LGIEMFVLDDGWFGHR AGA1_PEDPE 360 13
LGIELFVLDDGWFGHR AGAL_STRMU 352 13
LGVERFIIDDGWFKGR RAFA_ECOLI 321 13
LGLQMLVLDDGWFVNR AGA2_PEDPE 352 13
GLHYDRLASE365 Length of motif = 23 Motif number = 5
Glycosyl hydrolase family 36 motif V - 1
PCODE ST INT
FGIWLEPEMISYDSKLYQQHPDY AGA1_PEDPE 410 34
FGLWFEPEMVSVDSKLYRAHPDW AGAL_STRMU 402 34
FGIWVEPEMINPDSDLFRLHPDW RAFA_ECOLI 371 34
FGLWVEPEMITTNSQLYQQHPDW AGA2_PEDPE 402 34
GLHYDRLASE366 Length of motif = 16 Motif number = 6
Glycosyl hydrolase family 36 motif VI - 1
PCODE ST INT
LLKSKQIDYIKWDMNR AGA1_PEDPE 468 35
LLTENTIDYVKWDYNR AGAL_STRMU 460 35
LLGEHPVDYVKWDMNR RAFA_ECOLI 429 35
LVQNNQLDYLKWDMNR AGA2_PEDPE 460 35
GLHYDRLASE367 Length of motif = 17 Motif number = 7
Glycosyl hydrolase family 36 motif VII - 1
PCODE ST INT
HILFEGCSGGGGRFDAG AGA1_PEDPE 522 38
KVLFESCSGGGGRNDLG AGAL_STRMU 513 37
HVEFESCASGGGRIDFE RAFA_ECOLI 477 32
KLIIENCSAGGGRFDFG AGA2_PEDPE 514 38
GLHYDRLASE368 Length of motif = 17 Motif number = 8
Glycosyl hydrolase family 36 motif VIII - 1
PCODE ST INT
WASDNTDAVARLTIQYG AGA1_PEDPE 547 8
WSSDNTDAIARLPIQYG AGAL_STRMU 538 8
WASDNNDALERCTIQRG RAFA_ECOLI 502 8
WISDLTDPVDRATIENG AGA2_PEDPE 539 8
FINAL MOTIF SETS
GLHYDRLASE361 Length of motif = 16 Motif number = 1
Glycosyl hydrolase family 36 motif I - 2
PCODE ST INT
EFSGDAYGFNLVYSGN AGA1_PEDPE 243 243
EFYGEALALQLIYSGN AGAL_STRMU 235 235
EQQGEVWAVHLGWSGN RAFA_ECOLI 205 205
ESQGEAWGFSLVYTGS Q92457 257 257
ELAGTVIGCALAWSGN AGA2_PEDPE 239 239
GLHYDRLASE362 Length of motif = 21 Motif number = 2
Glycosyl hydrolase family 36 motif II - 2
PCODE ST INT
TPEVLMVYTNKGLNAMSQAYH AGA1_PEDPE 294 35
TPVALITYTDKGLTDLTQESH AGAL_STRMU 286 35
TPWLYACHSADGLNGMSQQYH RAFA_ECOLI 256 35
SPEAVAVFSTTGVGGMSRKFH Q92457 308 35
TPEAVLTWTNTGFNGMSQVFH AGA2_PEDPE 290 35
GLHYDRLASE363 Length of motif = 15 Motif number = 3
Glycosyl hydrolase family 36 motif III - 2
PCODE ST INT
PIVVNNWEATFFDFN AGA1_PEDPE 332 17
PILINNWEATYFDFN AGAL_STRMU 324 17
PVHLNTWEGIYFNHN RAFA_ECOLI 293 16
PVLLNSWEGLGFDYN Q92457 346 17
PSVLNTWETLTFAVS AGA2_PEDPE 324 13
GLHYDRLASE364 Length of motif = 16 Motif number = 4
Glycosyl hydrolase family 36 motif IV - 2
PCODE ST INT
LGIEMFVLDDGWFGHR AGA1_PEDPE 360 13
LGIELFVLDDGWFGHR AGAL_STRMU 352 13
LGVERFIIDDGWFKGR RAFA_ECOLI 321 13
LGIKLFVLDDGWFGVK Q92457 374 13
LGLQMLVLDDGWFVNR AGA2_PEDPE 352 13
GLHYDRLASE365 Length of motif = 23 Motif number = 5
Glycosyl hydrolase family 36 motif V - 2
PCODE ST INT
FGIWLEPEMISYDSKLYQQHPDY AGA1_PEDPE 410 34
FGLWFEPEMVSVDSKLYRAHPDW AGAL_STRMU 402 34
FGIWVEPEMINPDSDLFRLHPDW RAFA_ECOLI 371 34
FGLWFEPEMVNPNSTLYMEHPDW Q92457 434 44
FGLWVEPEMITTNSQLYQQHPDW AGA2_PEDPE 402 34
GLHYDRLASE366 Length of motif = 16 Motif number = 6
Glycosyl hydrolase family 36 motif VI - 2
PCODE ST INT
LLKSKQIDYIKWDMNR AGA1_PEDPE 468 35
LLTENTIDYVKWDYNR AGAL_STRMU 460 35
LLGEHPVDYVKWDMNR RAFA_ECOLI 429 35
ILSNASISYVKWDNNR Q92457 492 35
LVQNNQLDYLKWDMNR AGA2_PEDPE 460 35
GLHYDRLASE367 Length of motif = 17 Motif number = 7
Glycosyl hydrolase family 36 motif VII - 2
PCODE ST INT
HILFEGCSGGGGRFDAG AGA1_PEDPE 522 38
KVLFESCSGGGGRNDLG AGAL_STRMU 513 37
HVEFESCASGGGRIDFE RAFA_ECOLI 477 32
NVRWEGCASGGGRFDPG Q92457 538 30
KLIIENCSAGGGRFDFG AGA2_PEDPE 514 38
GLHYDRLASE368 Length of motif = 17 Motif number = 8
Glycosyl hydrolase family 36 motif VIII - 2
PCODE ST INT
WASDNTDAVARLTIQYG AGA1_PEDPE 547 8
WSSDNTDAIARLPIQYG AGAL_STRMU 538 8
WASDNNDALERCTIQRG RAFA_ECOLI 502 8
WTSDDTDAVERIAIQFG Q92457 563 8
WISDLTDPVDRATIENG AGA2_PEDPE 539 8
User query: Display/Full Code "GLHYDRLASE36"