WORKLIST ENTRIES (1):

GIPRECEPTOR View alignment      Gastric inhibitory polypeptide receptor precursor signature
 Type of fingerprint: COMPOUND with 11 elements
Links:
   PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
   PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
   PRINTS; PR00251 BACTRLOPSIN
   INTERPRO; IPR001749

 Creation date 22-MAY-1999

   1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
   Fingerprinting G protein-coupled receptors.
   PROTEIN ENG. 7(2) 195-203 (1994). 

   2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
   AND NAGATA, S.
   Molecular cloning and expression of a cDNA encoding the secretin receptor
   EMBO J. 10 1635-1641 (1991).
   
   3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H., 
   GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
   Expression cloning of adenylate cyclase-coupled calcitonin receptor
   SCIENCE 254 1022-1024 (1991).

   4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F., 
   SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
   KRONENBERG, H.M. AND SEGRE, G.E.
   A G protein linked receptor for parathyroid hormone and parathyroid
   hormone-related peptide.
   SCIENCE 254 1024-1026 (1991).

   5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
   Functional expression and tissue distribution of a novel receptor for
   vasoactive intestinal polypeptide.
   NEURON 8(4) 811-819 (1992).

   6. VOLZ, A., GOKE, R., LANKAT-BUTTGEREIT, B., FEHMANN, H.C., BODE, H.P.
   AND GOKE, B.
   Molecular cloning, functional expression, and signal transduction of the 
   GIP-receptor cloned from a human insulinoma.
   FEBS LETT. 373(1) 23-9 (1995).

   G protein-coupled receptors (GPCRs) constitute a vast protein family that 
   encompasses a wide range of functions (including various autocrine, para-
   crine and endocrine processes). They show considerable diversity at the 
   sequence level, on the basis of which they can be separated into distinct 
   groups. We use the term clan to describe the GPCRs, as they embrace a group
   of families for which there are indications of evolutionary relationship, 
   but between which there is no statistically significant similarity in 
   sequence [1]. The currently known clan members include the rhodopsin-like 
   GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
   pheromone receptors, and the metabotropic glutamate receptor family.
   
   The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid
   hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
   peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-
   inositol-calcium pathway. The amino acid sequences of the receptors contain
   high proportions of hydrophobic residues grouped into 7 domains, in a
   manner reminiscent of the rhodopsins and other receptors believed to inter-
   act with G proteins. However, while a similar 3D framework has been
   proposed to account for this, there is no significant sequence similarity
   between these families: the secretin-like receptors thus bear their own
   unique '7TM' signature.
  
   Glucose-dependent insulinotropic polypeptide (GIP) plays an important role
   in the regulation of postprandial insulin secretion and proinsulin gene 
   expression of pancreatic beta-cells [6]. The human GIP-receptor encodes a
   7TM protein that is similar to the human glucagon-like peptide 1(GLP-1) 
   receptor. It is hoped that an understanding of GIP-receptor regulation and
   signal transduction will shed light on the hormone's failure to exert its
   biological action at the pancreatic B-cell in type II diabetes mellitus. 
  
   GIPRECEPTOR is an 11-element fingerprint that provides a signature for  
   gastric inhibitory polypeptide receptors. The fingerprint was derived from
   an initial alignment of 3 sequences: the motifs were drawn from conserved
   regions spanning the full alignment length, focusing on those sections
   that characterise the gastric inhibitory polypeptide receptors but 
   distinguish them from the rest of the secretin-like superfamily - motifs 1-6
   span the N-terminal domain; motif 7 resides in the loop between TM domains 2
   and 3; motif 8 spans the loop between TM domains 3 and 4; motif 9 spans the C-terminal portion of TM domain 6 and
   loop between TM domains 4 and 5; and motifs 10 and 11 reside at the 
   C-terminus. A single iteration on SPTR37_9f was required to reach convergence,
   no further sequences being identified beyond the starting set. Two partial 
   matches were also found, secretin and glucagon receptors that match motifs
   1, 8 and 9.

  SUMMARY INFORMATION
      3 codes involving 11 elements
      0 codes involving 10 elements
      0 codes involving  9 elements
      0 codes involving  8 elements
      0 codes involving  7 elements
      0 codes involving  6 elements
      0 codes involving  5 elements
      0 codes involving  4 elements
      0 codes involving  3 elements
      2 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
   11|   3    3    3    3    3    3    3    3    3    3    3  
   10|   0    0    0    0    0    0    0    0    0    0    0  
    9|   0    0    0    0    0    0    0    0    0    0    0  
    8|   0    0    0    0    0    0    0    0    0    0    0  
    7|   0    0    0    0    0    0    0    0    0    0    0  
    6|   0    0    0    0    0    0    0    0    0    0    0  
    5|   0    0    0    0    0    0    0    0    0    0    0  
    4|   0    0    0    0    0    0    0    0    0    0    0  
    3|   0    0    0    0    0    0    0    0    0    0    0  
    2|   1    0    0    0    0    0    0    1    2    0    0  
   --+--------------------------------------------------------
     |   1    2    3    4    5    6    7    8    9   10   11  

True positives..
 GIPR_MESAU     GIPR_RAT       GIPR_HUMAN     
Subfamily:  Codes involving 2 elements
 Subfamily True positives..
 SCRC_RAT       GLR_RAT        


  PROTEIN TITLES
   GIPR_MESAU       GASTRIC INHIBITORY POLYPEPTIDE RECEPTOR PRECURSOR (GIP-R) (G
   GIPR_RAT         GASTRIC INHIBITORY POLYPEPTIDE RECEPTOR PRECURSOR (GIP-R) (G
   GIPR_HUMAN       GASTRIC INHIBITORY POLYPEPTIDE RECEPTOR PRECURSOR (GIP-R) (G
 
   SCRC_RAT         SECRETIN RECEPTOR PRECURSOR (SCT-R) - RATTUS NORVEGICUS (RAT
   GLR_RAT          GLUCAGON RECEPTOR PRECURSOR (GL-R) - RATTUS NORVEGICUS (RAT)

SCAN HISTORY SPTR37_9f 1 77 NSINGLE INITIAL MOTIF SETS GIPRECEPTOR1 Length of motif = 21 Motif number = 1 Gastric inhibitory polypeptide receptor precursor motif I - 1 PCODE ST INT PILQLLLRLSLCGLLLQRAET GIPR_HUMAN 5 5 PLRPRLLLLCLWGLLLQQAET GIPR_MESAU 2 2 PLRLLLLLLWLWGLSLQRAET GIPR_RAT 2 2 GIPRECEPTOR2 Length of motif = 18 Motif number = 2 Gastric inhibitory polypeptide receptor precursor motif II - 1 PCODE ST INT TGSKGQTAGELYQRWERY GIPR_HUMAN 25 -1 TDSEGQTTGELYQRWERY GIPR_MESAU 22 -1 TDSEGQTTGELYQRWERY GIPR_RAT 22 -1 GIPRECEPTOR3 Length of motif = 15 Motif number = 3 Gastric inhibitory polypeptide receptor precursor motif III - 1 PCODE ST INT CQETLAAAEPPSGLA GIPR_HUMAN 46 3 CEETLTAADPPSGMV GIPR_MESAU 43 3 CQNTLEATEPPSGLA GIPR_RAT 43 3 GIPRECEPTOR4 Length of motif = 14 Motif number = 4 Gastric inhibitory polypeptide receptor precursor motif IV - 1 PCODE ST INT HHVAAGFVLRQCGS GIPR_HUMAN 92 31 RHVAAGYVFRQCGS GIPR_MESAU 89 31 RQVAAGFVFRQCGS GIPR_RAT 89 31 GIPRECEPTOR5 Length of motif = 17 Motif number = 5 Gastric inhibitory polypeptide receptor precursor motif V - 1 PCODE ST INT WGLWRDHTQCENPEKNE GIPR_HUMAN 109 3 WGPWRDHTQCENPEKNG GIPR_MESAU 106 3 WGSWRDHTQCENPEKNG GIPR_RAT 106 3 GIPRECEPTOR6 Length of motif = 14 Motif number = 6 Gastric inhibitory polypeptide receptor precursor motif VI - 1 PCODE ST INT EAFLDQRLILERLQ GIPR_HUMAN 125 -1 GAFQDQRLILERLQ GIPR_MESAU 122 -1 GAFQDQKLILERLQ GIPR_RAT 122 -1 GIPRECEPTOR7 Length of motif = 21 Motif number = 7 Gastric inhibitory polypeptide receptor precursor motif VII - 1 PCODE ST INT PRPGPYLGDQALALWNQALAA GIPR_HUMAN 195 56 PTLGPYPGDRTLTLRNQALAA GIPR_MESAU 192 56 PPLGPYTGNQTPTLWNQALAA GIPR_RAT 192 56 GIPRECEPTOR8 Length of motif = 18 Motif number = 8 Gastric inhibitory polypeptide receptor precursor motif VIII - 1 PCODE ST INT HSLLVLVGGSEEGHFRYY GIPR_HUMAN 242 26 HHLLVIVGGSEKGHFRCY GIPR_MESAU 239 26 HHLLVVVRRSEKGHFRCY GIPR_RAT 239 26 GIPRECEPTOR9 Length of motif = 18 Motif number = 9 Gastric inhibitory polypeptide receptor precursor motif IX - 1 PCODE ST INT YLYENTQCWERNEVKAIW GIPR_HUMAN 279 19 YLLENTQCWERNEVKAIW GIPR_MESAU 276 19 YLYENTQCWERNEVKAIW GIPR_RAT 276 19 GIPRECEPTOR10 Length of motif = 18 Motif number = 10 Gastric inhibitory polypeptide receptor precursor motif X - 1 PCODE ST INT QLPERAFRALPSGSGPGE GIPR_HUMAN 422 125 PHAELGPQALPSRSAPRE GIPR_MESAU 420 126 PHLGQAPRAVPLSSAPQE GIPR_RAT 414 120 GIPRECEPTOR11 Length of motif = 17 Motif number = 11 Gastric inhibitory polypeptide receptor precursor motif XI - 1 PCODE ST INT LSSGTLPGPGNEASREL GIPR_HUMAN 446 6 LPSGPLHGPGEEVLESY GIPR_MESAU 445 7 LPSGMLHVPGDEVLESY GIPR_RAT 438 6 FINAL MOTIF SETS GIPRECEPTOR1 Length of motif = 21 Motif number = 1 Gastric inhibitory polypeptide receptor precursor motif I - 1 PCODE ST INT PLRPRLLLLCLWGLLLQQAET GIPR_MESAU 2 2 PLRLLLLLLWLWGLSLQRAET GIPR_RAT 2 2 PILQLLLRLSLCGLLLQRAET GIPR_HUMAN 5 5 GIPRECEPTOR2 Length of motif = 18 Motif number = 2 Gastric inhibitory polypeptide receptor precursor motif II - 1 PCODE ST INT TDSEGQTTGELYQRWERY GIPR_MESAU 22 -1 TDSEGQTTGELYQRWERY GIPR_RAT 22 -1 TGSKGQTAGELYQRWERY GIPR_HUMAN 25 -1 GIPRECEPTOR3 Length of motif = 15 Motif number = 3 Gastric inhibitory polypeptide receptor precursor motif III - 1 PCODE ST INT CEETLTAADPPSGMV GIPR_MESAU 43 3 CQNTLEATEPPSGLA GIPR_RAT 43 3 CQETLAAAEPPSGLA GIPR_HUMAN 46 3 GIPRECEPTOR4 Length of motif = 14 Motif number = 4 Gastric inhibitory polypeptide receptor precursor motif IV - 1 PCODE ST INT RHVAAGYVFRQCGS GIPR_MESAU 89 31 RQVAAGFVFRQCGS GIPR_RAT 89 31 HHVAAGFVLRQCGS GIPR_HUMAN 92 31 GIPRECEPTOR5 Length of motif = 17 Motif number = 5 Gastric inhibitory polypeptide receptor precursor motif V - 1 PCODE ST INT WGPWRDHTQCENPEKNG GIPR_MESAU 106 3 WGSWRDHTQCENPEKNG GIPR_RAT 106 3 WGLWRDHTQCENPEKNE GIPR_HUMAN 109 3 GIPRECEPTOR6 Length of motif = 14 Motif number = 6 Gastric inhibitory polypeptide receptor precursor motif VI - 1 PCODE ST INT GAFQDQRLILERLQ GIPR_MESAU 122 -1 GAFQDQKLILERLQ GIPR_RAT 122 -1 EAFLDQRLILERLQ GIPR_HUMAN 125 -1 GIPRECEPTOR7 Length of motif = 21 Motif number = 7 Gastric inhibitory polypeptide receptor precursor motif VII - 1 PCODE ST INT PTLGPYPGDRTLTLRNQALAA GIPR_MESAU 192 56 PPLGPYTGNQTPTLWNQALAA GIPR_RAT 192 56 PRPGPYLGDQALALWNQALAA GIPR_HUMAN 195 56 GIPRECEPTOR8 Length of motif = 18 Motif number = 8 Gastric inhibitory polypeptide receptor precursor motif VIII - 1 PCODE ST INT HHLLVIVGGSEKGHFRCY GIPR_MESAU 239 26 HHLLVVVRRSEKGHFRCY GIPR_RAT 239 26 HSLLVLVGGSEEGHFRYY GIPR_HUMAN 242 26 GIPRECEPTOR9 Length of motif = 18 Motif number = 9 Gastric inhibitory polypeptide receptor precursor motif IX - 1 PCODE ST INT YLLENTQCWERNEVKAIW GIPR_MESAU 276 19 YLYENTQCWERNEVKAIW GIPR_RAT 276 19 YLYENTQCWERNEVKAIW GIPR_HUMAN 279 19 GIPRECEPTOR10 Length of motif = 18 Motif number = 10 Gastric inhibitory polypeptide receptor precursor motif X - 1 PCODE ST INT PHAELGPQALPSRSAPRE GIPR_MESAU 420 126 PHLGQAPRAVPLSSAPQE GIPR_RAT 414 120 QLPERAFRALPSGSGPGE GIPR_HUMAN 422 125 GIPRECEPTOR11 Length of motif = 17 Motif number = 11 Gastric inhibitory polypeptide receptor precursor motif XI - 1 PCODE ST INT LPSGPLHGPGEEVLESY GIPR_MESAU 445 7 LPSGMLHVPGDEVLESY GIPR_RAT 438 6 LSSGTLPGPGNEASREL GIPR_HUMAN 446 6

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