WORKLIST ENTRIES (1):
GHSRECEPTOR View alignment View Structure Growth hormone secretagogue receptor type 1 signature
Type of fingerprint: COMPOUND with 10 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
Creation date 23-SEP-2000
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. MCKEE, K.K., PALYHA, O.C., FEIGHNER, S.D., HRENIUK, D.L., TAN, C.P.,
PHILLIPS, M.S., SMITH, R.G., VAN DER PLOEG, L.H. AND HOWARD, A.D.
Molecular analysis of rat pituitary and hypothalamic growth hormone
secretagogue receptors.
MOL.ENDOCRINOL. 11 415-423 (1997).
7. HOWARD, A.D., FEIGHNER, S.D., CULLY, D.F., ARENA, J.P., LIBERATOR, P.A.,
ROSENBLUM, C.I., HAMELIN, M., HRENIUK, D.L., PALYHA, O.C., ANDERSON, J.,
PARESS, P.S., DIAZ, C., CHOU, M., LIU, K.K., MCKEE, K.K., PONG, S.-S.,
CHAUNG, L.-Y., ELBRECHT, A., DASHKEVICZ, M., HEAVENS, R., RIGBY, M.,
SIRINATHSINGHJI, D.J.S., DEAN, D.C., MELILLO, D.G., PATCHETT, A.A.,
NARGUND, R., GRIFFIN, P.R., DEMARTINO, J.A., GUPTA, S.K., SCHAEFFER, J.M.,
SMITH, R.G. AND VAN DER PLOEG, L.H.T.
A receptor in pituitary and hypothalamus that functions in growth hormone
release.
SCIENCE 273 974-977 (1996).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Growth hormone (GH) release is thought to occur under the reciprocal
regulation of two hypothalamic peptides (GH releasing hormone (GHRH) and
somatostatin) via their engagement with specific cell surface receptors on
the anterior pituitary somatotroph [6,7]. GH-releasing peptides (such as
GHRP-6, and the nonpeptide mimetics, L-692, 429 and MK-0677) stimulate GH
release through their activation of a distinct receptor, the GH secretagogue
receptor (GHSR). This receptor couples to G-alpha-11 proteins.
GHSRECEPTOR is a 10-element fingerprint that provides a signature for the
growth hormone secretagogue type 1 receptors. The fingerprint was derived
from an initial alignment of 3 sequences: the motifs were drawn from
conserved sections within either loop or TM regions, focusing on those
areas of the alignment that characterise the growth hormone secretagogue
type 1 receptors but distinguish them from the rest of the rhodopsin-like
superfamily - motifs 1-3 span the N-terminal region, leading into TM domain
1; motif 4 encompasses part of the first external loop and TM domain 3;
motif 5 spans part of the second cytoplasmic loop and the N-terminal portion
of TM domain 4; motif 6 lies in the second cytoplasmic loop; motif 7
includes part of the second external loop and the N-terminus of TM domain 5;
motifs 8 and 9 spans the third cytoplasmic and third external loops
respectively; and motif 10 resides at the C-terminus. A single iteration on
SPTR37_10f was required to reach convergence, no further sequences being
identified beyond the starting set. Two partial matches were found: O93412
is an orphan GPCR from Sphoeroides nephelus that matches motifs 4, 7 and 9;
and GP38_HUMAN is a putative GPCR from Homo sapiens that matches motifs 3
and 4.
SUMMARY INFORMATION
3 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
1 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
10| 3 3 3 3 3 3 3 3 3 3
9| 0 0 0 0 0 0 0 0 0 0
8| 0 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0 0 0
3| 0 0 0 1 0 0 1 0 1 0
2| 0 0 1 1 0 0 0 0 0 0
--+---------------------------------------------------
| 1 2 3 4 5 6 7 8 9 10
True positives..
GHSR_RAT GHSR_HUMAN GHSR_PIG
Subfamily: Codes involving 3 elements
Subfamily True positives..
O93412
Subfamily: Codes involving 2 elements
Subfamily True positives..
GP38_HUMAN
PROTEIN TITLES
GHSR_RAT GROWTH HORMONE SECRETAGOGUE RECEPTOR TYPE 1 (GHS-R) - RATTUS
GHSR_HUMAN GROWTH HORMONE SECRETAGOGUE RECEPTOR TYPE 1 (GHS-R) - HOMO S
GHSR_PIG GROWTH HORMONE SECRETAGOGUE RECEPTOR TYPE 1 (GHS-R) - SUS SC
O93412 ORPHAN G PROTEIN-COUPLED RECEPTOR - SPHOEROIDES NEPHELUS.
GP38_HUMAN PUTATIVE G PROTEIN-COUPLED RECEPTOR GPR38 - HOMO SAPIENS (HU
SCAN HISTORY
SPTR37_10f 1 40 NSINGLE
INITIAL MOTIF SETS
GHSRECEPTOR1 Length of motif = 15 Motif number = 1
Growth hormone secretagogue receptor type 1 motif I - 1
PCODE ST INT
WNATPSEEPGFNLTL GHSR_HUMAN 2 2
WNATPSEEPGPNLTL GHSR_PIG 2 2
WNATPSEEPEPNVTL GHSR_RAT 2 2
GHSRECEPTOR2 Length of motif = 18 Motif number = 2
Growth hormone secretagogue receptor type 1 motif II - 1
PCODE ST INT
DLDWDASPGNDSLGDELL GHSR_HUMAN 18 1
DLGWDAPPENDSLVEELL GHSR_PIG 18 1
DLDWDASPGNDSLPDELL GHSR_RAT 17 0
GHSRECEPTOR3 Length of motif = 18 Motif number = 3
Growth hormone secretagogue receptor type 1 motif III - 1
PCODE ST INT
LFPAPLLAGVTATCVALF GHSR_HUMAN 37 1
LFPTPLLAGVTATCVALF GHSR_PIG 37 1
LFPAPLLAGVTATCVALF GHSR_RAT 36 1
GHSRECEPTOR4 Length of motif = 17 Motif number = 4
Growth hormone secretagogue receptor type 1 motif IV - 1
PCODE ST INT
FQFVSESCTYATVLTIT GHSR_HUMAN 119 64
FQFVSESCTYATVLTIT GHSR_PIG 119 64
FQFVSESCTYATVLTIT GHSR_RAT 118 64
GHSRECEPTOR5 Length of motif = 14 Motif number = 5
Growth hormone secretagogue receptor type 1 motif V - 1
PCODE ST INT
AKVVVTKGRVKLVI GHSR_HUMAN 151 15
AKVVVTKGRVKLVI GHSR_PIG 151 15
AKVVVTKGRVKLVI GHSR_RAT 150 15
GHSRECEPTOR6 Length of motif = 15 Motif number = 6
Growth hormone secretagogue receptor type 1 motif VI - 1
PCODE ST INT
GVEHENGTDPWDTNE GHSR_HUMAN 183 18
GVEHDNGTDPRDTNE GHSR_PIG 183 18
GVEHENGTDPRDTNE GHSR_RAT 182 18
GHSRECEPTOR7 Length of motif = 15 Motif number = 7
Growth hormone secretagogue receptor type 1 motif VII - 1
PCODE ST INT
TEFAVRSGLLTVMVW GHSR_HUMAN 201 3
TEFAVRSGLLTVMVW GHSR_PIG 201 3
TEFAVRSGLLTVMVW GHSR_RAT 200 3
GHSRECEPTOR8 Length of motif = 20 Motif number = 8
Growth hormone secretagogue receptor type 1 motif VIII - 1
PCODE ST INT
RGDAVVGASLRDQNHKQTVK GHSR_HUMAN 244 28
RGEAAVGSSLRDQNHKQTVK GHSR_PIG 244 28
RGDAAVGASLRDQNHKQTVK GHSR_RAT 242 27
GHSRECEPTOR9 Length of motif = 21 Motif number = 9
Growth hormone secretagogue receptor type 1 motif IX - 1
PCODE ST INT
FHVGRYLFSKSFEPGSLEIAQ GHSR_HUMAN 279 15
FHVGRYLFSKSLEPGSVEIAQ GHSR_PIG 279 15
FHVGRYLFSKSFEPGSLEIAQ GHSR_RAT 277 15
GHSRECEPTOR10 Length of motif = 23 Motif number = 10
Growth hormone secretagogue receptor type 1 motif X - 1
PCODE ST INT
FSQRKLSTLKDESSRAWTESSIN GHSR_HUMAN 343 43
FSQRKLSTLKDESSRAWTESSIN GHSR_PIG 343 43
FSQRKLSTLKDESSRAWTKSSIN GHSR_RAT 341 43
FINAL MOTIF SETS
GHSRECEPTOR1 Length of motif = 15 Motif number = 1
Growth hormone secretagogue receptor type 1 motif I - 1
PCODE ST INT
WNATPSEEPEPNVTL GHSR_RAT 2 2
WNATPSEEPGFNLTL GHSR_HUMAN 2 2
WNATPSEEPGPNLTL GHSR_PIG 2 2
GHSRECEPTOR2 Length of motif = 18 Motif number = 2
Growth hormone secretagogue receptor type 1 motif II - 1
PCODE ST INT
DLDWDASPGNDSLPDELL GHSR_RAT 17 0
DLDWDASPGNDSLGDELL GHSR_HUMAN 18 1
DLGWDAPPENDSLVEELL GHSR_PIG 18 1
GHSRECEPTOR3 Length of motif = 18 Motif number = 3
Growth hormone secretagogue receptor type 1 motif III - 1
PCODE ST INT
LFPAPLLAGVTATCVALF GHSR_RAT 36 1
LFPAPLLAGVTATCVALF GHSR_HUMAN 37 1
LFPTPLLAGVTATCVALF GHSR_PIG 37 1
GHSRECEPTOR4 Length of motif = 17 Motif number = 4
Growth hormone secretagogue receptor type 1 motif IV - 1
PCODE ST INT
FQFVSESCTYATVLTIT GHSR_RAT 118 64
FQFVSESCTYATVLTIT GHSR_HUMAN 119 64
FQFVSESCTYATVLTIT GHSR_PIG 119 64
GHSRECEPTOR5 Length of motif = 14 Motif number = 5
Growth hormone secretagogue receptor type 1 motif V - 1
PCODE ST INT
AKVVVTKGRVKLVI GHSR_RAT 150 15
AKVVVTKGRVKLVI GHSR_HUMAN 151 15
AKVVVTKGRVKLVI GHSR_PIG 151 15
GHSRECEPTOR6 Length of motif = 15 Motif number = 6
Growth hormone secretagogue receptor type 1 motif VI - 1
PCODE ST INT
GVEHENGTDPRDTNE GHSR_RAT 182 18
GVEHENGTDPWDTNE GHSR_HUMAN 183 18
GVEHDNGTDPRDTNE GHSR_PIG 183 18
GHSRECEPTOR7 Length of motif = 15 Motif number = 7
Growth hormone secretagogue receptor type 1 motif VII - 1
PCODE ST INT
TEFAVRSGLLTVMVW GHSR_RAT 200 3
TEFAVRSGLLTVMVW GHSR_HUMAN 201 3
TEFAVRSGLLTVMVW GHSR_PIG 201 3
GHSRECEPTOR8 Length of motif = 20 Motif number = 8
Growth hormone secretagogue receptor type 1 motif VIII - 1
PCODE ST INT
RGDAAVGASLRDQNHKQTVK GHSR_RAT 242 27
RGDAVVGASLRDQNHKQTVK GHSR_HUMAN 244 28
RGEAAVGSSLRDQNHKQTVK GHSR_PIG 244 28
GHSRECEPTOR9 Length of motif = 21 Motif number = 9
Growth hormone secretagogue receptor type 1 motif IX - 1
PCODE ST INT
FHVGRYLFSKSFEPGSLEIAQ GHSR_RAT 277 15
FHVGRYLFSKSFEPGSLEIAQ GHSR_HUMAN 279 15
FHVGRYLFSKSLEPGSVEIAQ GHSR_PIG 279 15
GHSRECEPTOR10 Length of motif = 23 Motif number = 10
Growth hormone secretagogue receptor type 1 motif X - 1
PCODE ST INT
FSQRKLSTLKDESSRAWTKSSIN GHSR_RAT 341 43
FSQRKLSTLKDESSRAWTESSIN GHSR_HUMAN 343 43
FSQRKLSTLKDESSRAWTESSIN GHSR_PIG 343 43
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