WORKLIST ENTRIES (1):
DIUHORMONER View alignment Diuretic hormone receptor signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
INTERPRO; IPR002001
Creation date 22-MAY-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
AND NAGATA, S.
Molecular cloning and expression of a cDNA encoding the secretin receptor
EMBO J. 10 1635-1641 (1991).
3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H.,
GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
Expression cloning of adenylate cyclase-coupled calcitonin receptor
SCIENCE 254 1022-1024 (1991).
4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F.,
SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
KRONENBERG, H.M. AND SEGRE, G.E.
A G protein linked receptor for parathyroid hormone and parathyroid
hormone-related peptide.
SCIENCE 254 1024-1026 (1991).
5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
Functional expression and tissue distribution of a novel receptor for
vasoactive intestinal polypeptide.
NEURON 8(4) 811-819 (1992).
6. REAGAN, J.D.
Molecular cloning and function expression of a diuretic hormone receptor
from the house cricket, Acheta domesticus.
INSECT BIOCHEM.MOL.BIOL. 26(1) 1-6 (1996).
7. REAGAN, J.D.
Expression cloning of an insect diuretic hormone receptor. A member of the
calcitonin/secretin receptor family.
J.BIOL.CHEM. 269(1) 9-12 (1994).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid
hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-
inositol-calcium pathway. The amino acid sequences of the receptors contain
high proportions of hydrophobic residues grouped into 7 domains, in a
manner reminiscent of the rhodopsins and other receptors believed to inter-
act with G proteins. However, while a similar 3D framework has been
proposed to account for this, there is no significant sequence similarity
between these families: the secretin-like receptors thus bear their own
unique '7TM' signature.
Insect diuretic hormones regulate fluid and ion secretion, and the receptors
with which they interact are attractive targets for new insect control
agents [6]. Diuretic hormone receptors from the moth, Manduca sexta, and the
house cricket, Acheta domesticus, share 53% sequence identity and have been
shown to be members of the secretin-like family of GPCRs [7]. The receptors
bind diuretic hormone with high affinity and stimulate adenylate cyclase
with high potency.
DIUHORMONER is an 8-element fingerprint that provides a signature for
diuretic hormone receptors. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved regions
spanning the full alignment length, focusing on those sections that
characterise the diuretic hormone receptors but distinguish them from the
rest of the secretin-like superfamily - motifs 1 and 2 lie immediately
N-terminal to TM domain 1; motif 3 spans TM domain 2; motif 4 resides in
the loop between TM domains 3 and 4; motif 5 lies between TM domains 4 and
5; motif 6 spans the C-terminal portion of TM domain 6 and its following
loop; and motifs 7 and 8 reside at the C-terminus. A single iteration on
SPTR37_9f was required to reach convergence, no further sequences being
identified beyond the starting set.
SUMMARY INFORMATION
2 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 2 2 2 2 2 2 2 2
7| 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
DIHR_ACHDO DIHR_MANSE
PROTEIN TITLES
DIHR_ACHDO DIURETIC HORMONE RECEPTOR PRECURSOR (DH-R) - ACHETA DOMESTIC
DIHR_MANSE DIURETIC HORMONE RECEPTOR (DH-R) - MANDUCA SEXTA (TOBACCO HA
SCAN HISTORY
SPTR37_9f 1 100 NSINGLE
INITIAL MOTIF SETS
DIUHORMONER1 Length of motif = 20 Motif number = 1
Diuretic hormone receptor motif I - 1
PCODE ST INT
CFKELYGIQYDDTQNASRLC DIHR_MANSE 40 40
CFDELNGIRYDIRQNATRMC DIHR_ACHDO 85 85
DIUHORMONER2 Length of motif = 25 Motif number = 2
Diuretic hormone receptor motif II - 1
PCODE ST INT
GVWHNYTNYTNCTERIANGSPTDVA DIHR_MANSE 62 2
GTWRNYSDYVHCRELVEAESDEDAA DIHR_ACHDO 108 3
DIUHORMONER3 Length of motif = 17 Motif number = 3
Diuretic hormone receptor motif III - 1
PCODE ST INT
YILSACSWILNLVLQNW DIHR_MANSE 129 42
YICNDATWIISAVVQEY DIHR_ACHDO 176 43
DIUHORMONER4 Length of motif = 16 Motif number = 4
Diuretic hormone receptor motif IV - 1
PCODE ST INT
LVVETFTAENIKLKVY DIHR_MANSE 184 38
LVVATFTGEKVKLQIY DIHR_ACHDO 227 34
DIUHORMONER5 Length of motif = 16 Motif number = 5
Diuretic hormone receptor motif V - 1
PCODE ST INT
KMCIWMHEHQVDWIHK DIHR_MANSE 239 39
KHCPWMAEDYFDWIHQ DIHR_ACHDO 282 39
DIUHORMONER6 Length of motif = 14 Motif number = 6
Diuretic hormone receptor motif VI - 1
PCODE ST INT
GITNLLVLCGPSDD DIHR_MANSE 305 50
GITYILMMQGPMDG DIHR_ACHDO 348 50
DIUHORMONER7 Length of motif = 22 Motif number = 7
Diuretic hormone receptor motif VII - 1
PCODE ST INT
RYHVERWKTGRTIGGGRRRGAS DIHR_MANSE 356 37
RHRMSRWRETRTVGGGRRYTLS DIHR_ACHDO 398 36
DIUHORMONER8 Length of motif = 14 Motif number = 8
Diuretic hormone receptor motif VIII - 1
PCODE ST INT
SKDWSPRSRTESIR DIHR_MANSE 379 1
SKDWSPRSRTESIR DIHR_ACHDO 422 2
FINAL MOTIF SETS
DIUHORMONER1 Length of motif = 20 Motif number = 1
Diuretic hormone receptor motif I - 1
PCODE ST INT
CFDELNGIRYDIRQNATRMC DIHR_ACHDO 85 85
CFKELYGIQYDDTQNASRLC DIHR_MANSE 40 40
DIUHORMONER2 Length of motif = 25 Motif number = 2
Diuretic hormone receptor motif II - 1
PCODE ST INT
GTWRNYSDYVHCRELVEAESDEDAA DIHR_ACHDO 108 3
GVWHNYTNYTNCTERIANGSPTDVA DIHR_MANSE 62 2
DIUHORMONER3 Length of motif = 17 Motif number = 3
Diuretic hormone receptor motif III - 1
PCODE ST INT
YICNDATWIISAVVQEY DIHR_ACHDO 176 43
YILSACSWILNLVLQNW DIHR_MANSE 129 42
DIUHORMONER4 Length of motif = 16 Motif number = 4
Diuretic hormone receptor motif IV - 1
PCODE ST INT
LVVATFTGEKVKLQIY DIHR_ACHDO 227 34
LVVETFTAENIKLKVY DIHR_MANSE 184 38
DIUHORMONER5 Length of motif = 16 Motif number = 5
Diuretic hormone receptor motif V - 1
PCODE ST INT
KHCPWMAEDYFDWIHQ DIHR_ACHDO 282 39
KMCIWMHEHQVDWIHK DIHR_MANSE 239 39
DIUHORMONER6 Length of motif = 14 Motif number = 6
Diuretic hormone receptor motif VI - 1
PCODE ST INT
GITYILMMQGPMDG DIHR_ACHDO 348 50
GITNLLVLCGPSDD DIHR_MANSE 305 50
DIUHORMONER7 Length of motif = 22 Motif number = 7
Diuretic hormone receptor motif VII - 1
PCODE ST INT
RHRMSRWRETRTVGGGRRYTLS DIHR_ACHDO 398 36
RYHVERWKTGRTIGGGRRRGAS DIHR_MANSE 356 37
DIUHORMONER8 Length of motif = 14 Motif number = 8
Diuretic hormone receptor motif VIII - 1
PCODE ST INT
SKDWSPRSRTESIR DIHR_ACHDO 422 2
SKDWSPRSRTESIR DIHR_MANSE 379 1
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