WORKLIST ENTRIES (1):
CLOACNIMMNTY View alignment View Structure Cloacin immunity protein signature
Type of fingerprint: COMPOUND with 4 elements
Links:
PRINTS; PR01295 CLOACIN; PR01297 LYSISCOLICIN; PR01298 MICROCIN
PRINTS; PR01299 PYOCIN; PR01300 PYOCINKILLER
Creation date 17-FEB-2000
1. VAN DEN ELZEN, P.J., GAASTRA, W., SPELT, C.E., DE GRAAF, F.K.,
VELTKAMP, E. AND NIJKAMP, H.J.
Molecular structure of the immunity gene and immunity protein of the
bacteriocinogenic plasmid Clo DF13.
NUCLEIC ACIDS RES. 8 4349-4363 (1980).
The cloacin immunity protein complexes with cloacin in equimolar quantities
and inhibits it by binding with high affinity to the cloacin C-terminal
catalytic domain. The immunity protein is relatively small, containing 85
amino acids.
An extra ribosome binding site has been found to precede the immunity gene
on the polycistronic Clo DF13 mRNA [1], which perhaps accounts for the fact
that, in cloacinogenic cells, more immunity protein than cloacin is
synthesised [1]. Comparison of the complete amino acid sequence of the Clo
DF13 immunity protein with that of the Col E3 and Col E6 immunity proteins
reveals extensive similarities in primary structure, although Col E3 and
Clo DF13 immunity proteins are exchangeable only to a low extent in vivo
and in vitro [1].
CLOACNIMMNTY is a 4-element fingerprint that provides a signature for
cloacin immunity proteins. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved regions
spanning the full alignment length. Two iterations on SPTR37_10f were
required to reach convergence, at which point a true set comprising 4
sequences was identified.
SUMMARY INFORMATION
4 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
4| 4 4 4 4
3| 0 0 0 0
2| 0 0 0 0
--+---------------------
| 1 2 3 4
True positives..
IMM6_ECOLI IMM3_ECOLI IMMC_ECOLI Q47110
PROTEIN TITLES
IMM6_ECOLI COLICIN E6 IMMUNITY PROTEIN (IMME6) (MICROCIN E6 IMMUNITY PR
IMM3_ECOLI COLICIN E3 IMMUNITY PROTEIN (IMME3) (MICROCIN E3 IMMUNITY PR
IMMC_ECOLI IMMUNITY PROTEIN FOR CLOACIN - ESCHERICHIA COLI.
Q47110 IMMUNITY PROTEIN - ESCHERICHIA COLI.
SCAN HISTORY
SPTR37_10f 1 100 NSINGLE
INITIAL MOTIF SETS
CLOACNIMMNTY1 Length of motif = 17 Motif number = 1
Cloacin immunity protein motif I - 1
PCODE ST INT
GLKLDLTWFDKSTEDFK IMM3_ECOLI 2 2
GLKLHINWFDKRTEEFK IMM6_ECOLI 2 2
GLKLHIHWFDKKTEEFK IMMC_ECOLI 2 2
CLOACNIMMNTY2 Length of motif = 21 Motif number = 2
Cloacin immunity protein motif II - 1
PCODE ST INT
GEEYSKDFGDDGSVMESLGVP IMM3_ECOLI 19 0
GGEYSKDFGDDGSVIERLGMP IMM6_ECOLI 19 0
GGEYSKDFGDDGSVIESLGMP IMMC_ECOLI 19 0
CLOACNIMMNTY3 Length of motif = 20 Motif number = 3
Cloacin immunity protein motif III - 1
PCODE ST INT
KDNVNNGCFDVIAEWVPLLQ IMM3_ECOLI 41 1
KDNINNGWFDVIAEWVPLLQ IMM6_ECOLI 41 1
KDNINNGWFDVEKPWVSILQ IMMC_ECOLI 41 1
CLOACNIMMNTY4 Length of motif = 23 Motif number = 4
Cloacin immunity protein motif IV - 1
PCODE ST INT
PYFNHQIDISDNEYFVSFDYRDG IMM3_ECOLI 61 0
PYFNHQIDISDNEYFVSFDYRDG IMM6_ECOLI 61 0
PHFKNVIDISKFDYFVSFVYRDG IMMC_ECOLI 61 0
FINAL MOTIF SETS
CLOACNIMMNTY1 Length of motif = 17 Motif number = 1
Cloacin immunity protein motif I - 2
PCODE ST INT
GLKLHINWFDKRTEEFK IMM6_ECOLI 2 2
GLKLDLTWFDKSTEDFK IMM3_ECOLI 2 2
GLKLHIHWFDKKTEEFK IMMC_ECOLI 2 2
GLKLNLTWFDKKTEDFK Q47110 2 2
CLOACNIMMNTY2 Length of motif = 21 Motif number = 2
Cloacin immunity protein motif II - 2
PCODE ST INT
GGEYSKDFGDDGSVIERLGMP IMM6_ECOLI 19 0
GEEYSKDFGDDGSVMESLGVP IMM3_ECOLI 19 0
GGEYSKDFGDDGSVIESLGMP IMMC_ECOLI 19 0
GEEYSKDFGDDGSVIESLGMP Q47110 19 0
CLOACNIMMNTY3 Length of motif = 20 Motif number = 3
Cloacin immunity protein motif III - 2
PCODE ST INT
KDNINNGWFDVIAEWVPLLQ IMM6_ECOLI 41 1
KDNVNNGCFDVIAEWVPLLQ IMM3_ECOLI 41 1
KDNINNGWFDVEKPWVSILQ IMMC_ECOLI 41 1
KDNINNGGFDVKKSWVPLLQ Q47110 41 1
CLOACNIMMNTY4 Length of motif = 23 Motif number = 4
Cloacin immunity protein motif IV - 2
PCODE ST INT
PYFNHQIDISDNEYFVSFDYRDG IMM6_ECOLI 61 0
PYFNHQIDISDNEYFVSFDYRDG IMM3_ECOLI 61 0
PHFKNVIDISKFDYFVSFVYRDG IMMC_ECOLI 61 0
PYFKNKIEVDKYWYQISFDYRDG Q47110 61 0
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