WORKLIST ENTRIES (1):
CANABINOID2R View alignment View Structure Cannabinoid receptor type 2 signature
Type of fingerprint: COMPOUND with 5 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00362 CANNABINOIDR; PR00522 CANABINOID1R
INTERPRO; IPR001551
Creation date 19-MAY-1996; UPDATE 06-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
Cannabinoid.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.80-83.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
The ability of marijuana to activate the cannabinoid receptor provides a
molecular explanation for its psychoactive effects and other CNS actions
(which include hallucinations, memory deficits, altered time and space
perception, CNS depression and appetite stimulation) [6]. The endogenous
ligand at the cannabinoid receptor is unknown, although it may be a
derivative of arachidonic acid. The cannabinoid receptor is widespread
throughout the CNS, high levels occurring in the dendate gyrus, hippocampus
and cerebral cortex, with more moderate levels in the hypothalamus and
amygdala. It is also present in various cell lines, and in the periphery
it is found in the testis and vas deferens [6].
CANABINOID2R is a 5-element fingerprint that provides a signature for type
2 cannabinoid receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise type 2 cannabinoid receptors but
distinguish them from the rest of the cannabinoid receptor family - motif 1
lies in the N-terminal region immediately preceding TM domain 1; motif 2
spans the third cytoplasmic loop; motif 3 spans the third external loop;
and motifs 4 and 5 lie in the C-terminal region following TM domain 7. A
single iteration on OWL27.1 was required to reach convergence, no further
sequences being identified beyond the starting set.
An update on SPTR37_9f identified a true set of 2 sequences.
SUMMARY INFORMATION
2 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
5| 2 2 2 2 2
4| 0 0 0 0 0
3| 0 0 0 0 0
2| 0 0 0 0 0
--+--------------------------
| 1 2 3 4 5
True positives..
CB2R_HUMAN CB2R_MOUSE
PROTEIN TITLES
CB2R_HUMAN CANNABINOID RECEPTOR 2 (CB2) (CB-2) (CX5) - HOMO SAPIENS (HU
CB2R_MOUSE CANNABINOID RECEPTOR 2 (CB2) (CB-2) - MUS MUSCULUS (MOUSE).
SCAN HISTORY
OWL27_1 1 100 NSINGLE
SPTR37_9f 2 3 NSINGLE
INITIAL MOTIF SETS
CANABINOID2R1 Length of motif = 19 Motif number = 1
Cannabinoid receptor 2 motif I - 1
PCODE ST INT
TEIANGSKDGLDSNPMKDY CB2R_HUMAN 7 7
TEVTNGSNGGLEFNPMKEY S54163 7 7
TEVTNGSNGGLEFNPMKEY MMU21681 7 7
CANABINOID2R2 Length of motif = 18 Motif number = 2
Cannabinoid receptor 2 motif II - 1
PCODE ST INT
VASLSGHQDRQVPGMARM CB2R_HUMAN 220 194
VATLAEHQDRQVPGIARM S54163 220 194
VATLAEHQDRQVPGIARM MMU21681 220 194
CANABINOID2R3 Length of motif = 17 Motif number = 3
Cannabinoid receptor 2 motif III - 1
PCODE ST INT
LMAHSLATTLSDQVKKA CB2R_HUMAN 264 26
LMGHSLVTTLSDQVKEA S54163 264 26
LMGHSLVTMLSDQVKEA MMU21681 264 26
CANABINOID2R4 Length of motif = 18 Motif number = 4
Cannabinoid receptor 2 motif IV - 1
PCODE ST INT
RSSAHHCLAHWKKCVRGL CB2R_HUMAN 307 26
RSAAQHCLIGWKKYLQGL S54163 307 26
RSAAQHCLIGWKKYLQGL MMU21681 307 26
CANABINOID2R5 Length of motif = 19 Motif number = 5
Cannabinoid receptor 2 motif V - 1
PCODE ST INT
GSEAKEEAPRSSVTETEAD CB2R_HUMAN 325 0
GPEGKEEGPRSSVTETEAD S54163 325 0
GPEGKEEAPRSSVTETEAD MMU21681 325 0
FINAL MOTIF SETS
CANABINOID2R1 Length of motif = 19 Motif number = 1
Cannabinoid receptor 2 motif I - 2
PCODE ST INT
TEIANGSKDGLDSNPMKDY CB2R_HUMAN 7 7
TEVTNGSNGGLEFNPMKEY CB2R_MOUSE 7 7
CANABINOID2R2 Length of motif = 18 Motif number = 2
Cannabinoid receptor 2 motif II - 2
PCODE ST INT
VASLSGHQDRQVPGMARM CB2R_HUMAN 220 194
VATLAEHQDRQVPGIARM CB2R_MOUSE 220 194
CANABINOID2R3 Length of motif = 17 Motif number = 3
Cannabinoid receptor 2 motif III - 2
PCODE ST INT
LMAHSLATTLSDQVKKA CB2R_HUMAN 264 26
LMGHSLVTTLSDQVKEA CB2R_MOUSE 264 26
CANABINOID2R4 Length of motif = 18 Motif number = 4
Cannabinoid receptor 2 motif IV - 2
PCODE ST INT
RSSAHHCLAHWKKCVRGL CB2R_HUMAN 307 26
RSAAQHCLIGWKKYLQGL CB2R_MOUSE 307 26
CANABINOID2R5 Length of motif = 19 Motif number = 5
Cannabinoid receptor 2 motif V - 2
PCODE ST INT
GSEAKEEAPRSSVTETEAD CB2R_HUMAN 325 0
GPEGKEEGPRSSVTETEAD CB2R_MOUSE 325 0
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