WORKLIST ENTRIES (1):

BUTYPHLNCDUF View alignment     Butyrophylin C-terminal DUF signature 
 Type of fingerprint: COMPOUND with 6  elements
Links:
   PRINTS; PR01406 BBOXZNFINGER
   PFAM; PF00622 SPRY
   INTERPRO; IPR000107

 Creation date 25-APR-2000

   1. BORDEN, K.L. 
   RING fingers and B-boxes: zinc-binding protein-protein interaction domains. 
   BIOCHEM.CELL BIOL. 76 351-358 (1998).

   2. BORDEN, K.L., LALLY, J.M., MARTIN, S.R., O'REILLY, N.J., ETKIN, L.D.
   AND FREEMONT, P.S.
   Novel topology of a zinc-binding domain from a protein involved in
   regulating early Xenopus development. 
   EMBO J. 14(23) 5947-5956 (1995).

   3. PONTING, C., SCHULTZ, J. AND BORK, P
   SPRY domains in ryanodine receptors (Ca(2+)-release channels).
   TRENDS BIOCHEM.SCI. 22 193-194 (1997).

   Several proteins that contain RING fingers also contain a well-conserved
   40-residue cysteine-rich domain termed a B-box zinc finger. Often, one or 
   two copies of the B-box are associated with a coiled coil domain in addition
   to the ring finger, forming a tripartite motif. The tripartite motif is 
   found in transcription factors, ribonucleoproteins and proto-oncoproteins,
   but no function has yet been ascribed to the domain [1]. 
  
   The solution structure of the B-box motif has been determined by NMR [2]. 
   The protein is a monomer, with 2 beta-strands, 2 helical turns and 3
   extended loop regions packed in a novel topology [2]. Of 7 potential zinc
   ligands, only 4 are used, binding a single zinc atom in a C2-H2 tetrahedral 
   arrangement [2]. The B-box structure differs in tertiary fold from all
   other known zinc-binding motifs.
  
   A group of proteins that contain the B-box motif also host a well conserved
   domain of unknown function (DUF). Proteins that include this domain are,
   e.g.: butyrophilin, the RET finger protein, the 52kDa Ro protein and the
   Xenopus nuclear factor protein. The C-terminal portion of this region has
   been termed the SPRY domain (after SPla and the RYanodine Receptor) [1].
  
   BUTYPHLNCDUF is a 6-element fingerprint that provides a signature for the
   butyrophilin C-terminal domain and related proteins. The fingerprint was
   derived from an initial alignment of 7 proteins: the motifs were drawn from
   short conserved regions spanning the C-terminal portion of the alignment -
   motifs 1-3 lie N-terminal to the SPRY domain, and motifs 4-6 reside within
   it. Five iterations on SPTR37_10f were required to reach convergence, at
   which point a true set comprising 26 sequences was identified. Several
   partial matches were also found, all of which are related DNA-binding
   proteins that fail to match one or more motifs.

  SUMMARY INFORMATION
     26 codes involving  6 elements
      0 codes involving  5 elements
      1 codes involving  4 elements
      2 codes involving  3 elements
      7 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    6|  26   26   26   26   26   26  
    5|   0    0    0    0    0    0  
    4|   0    0    1    1    1    1  
    3|   1    0    2    1    2    0  
    2|   2    0    6    0    4    2  
   --+-------------------------------
     |   1    2    3    4    5    6  

True positives..
 BUTY_HUMAN     BUTY_MOUSE     O00480         BUTY_BOVIN     
 O00475         O00478         RO52_HUMAN     O00481         
 O75657         P78408         RFP_HUMAN      RFP_MOUSE      
 P78409         O75658         O75656         O00635         
 O15553         O75677         RO52_MOUSE     O75679         
 O75678         A33_PLEWA      Q91431         Q92021         
 O95604         Q12899         
Subfamily:  Codes involving 4 elements
 Subfamily True positives..
 Q91885         
Subfamily:  Codes involving 3 elements
 Subfamily True positives..
 Q91884         O95361         
Subfamily:  Codes involving 2 elements
 Subfamily True positives..
 Y129_HUMAN     Q14258         Q61510         O70583         
 O35418         O15344         Q91453         


  PROTEIN TITLES
   BUTY_HUMAN       BUTYROPHILIN PRECURSOR (BT) - HOMO SAPIENS (HUMAN).
   BUTY_MOUSE       BUTYROPHILIN PRECURSOR (BT) - MUS MUSCULUS (MOUSE).
   O00480           BUTYROPHILIN (BTF2) (BUTYROPHILIN) - HOMO SAPIENS (HUMAN).
   BUTY_BOVIN       BUTYROPHILIN PRECURSOR (BT) - BOS TAURUS (BOVINE).
   O00475           BUTYROPHILIN - HOMO SAPIENS (HUMAN).
   O00478           BUTYROPHILIN - HOMO SAPIENS (HUMAN).
   RO52_HUMAN       52 KD RO PROTEIN (SJOGREN SYNDROME TYPE A ANTIGEN (SS-A)) (R
   O00481           BUTYROPHILIN - HOMO SAPIENS (HUMAN).
   O75657           BTF5 (BUTYROPHILIN) - HOMO SAPIENS (HUMAN).
   P78408           BUTYROPHILIN PROTEIN - HOMO SAPIENS (HUMAN).
   RFP_HUMAN        ZINC-FINGER PROTEIN RFP (RET FINGER PROTEIN) - HOMO SAPIENS 
   RFP_MOUSE        ZINC-FINGER PROTEIN RFP (RET FINGER PROTEIN) - MUS MUSCULUS 
   P78409           BUTYROPHILIN - HOMO SAPIENS (HUMAN).
   O75658           BTF4 (BUTYROPHILIN) - HOMO SAPIENS (HUMAN).
   O75656           DJ45P21.2 (BUTYROPHILIN) - HOMO SAPIENS (HUMAN).
   O00635           SIMILAR TO THE 52KD RO/SSA PROTEIN AND RFP - HOMO SAPIENS (H
   O15553           PYRIN - HOMO SAPIENS (HUMAN).
   O75677           RFPL1L - HOMO SAPIENS (HUMAN).
   RO52_MOUSE       52 KD RO PROTEIN (SJOGREN SYNDROME TYPE A ANTIGEN (SS-A)) (R
   O75679           RET FINGER PROTEIN-LIKE 3 - HOMO SAPIENS (HUMAN).
   O75678           RET FINGER PROTEIN-LIKE 2 - HOMO SAPIENS (HUMAN).
   A33_PLEWA        ZINC-BINDING PROTEIN A33 - PLEURODELES WALTLII (IBERIAN RIBB
   Q91431           NUCLEAR FACTOR 7 - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
   Q92021           XNF7=ZINC FINGER NUCLEAR PHOSPHOPROTEIN - XENOPUS LAEVIS (AF
   O95604           ZINC FINGER PROTEIN - HOMO SAPIENS (HUMAN).
   Q12899           ACID FINGER PROTEIN - HOMO SAPIENS (HUMAN).
 
   Q91885           RING FINGER PROTEIN - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
 
   Q91884           RING FINGER PROTEIN - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
   O95361           ESTROGEN-RESPONSIVE B BOX PROTEIN - HOMO SAPIENS (HUMAN).
 
   Y129_HUMAN       HYPOTHETICAL PROTEIN KIAA0129 - HOMO SAPIENS (HUMAN).
   Q14258           ESTROGEN RESPONSIVE FINGER PROTEIN (EFP) - HOMO SAPIENS (HUM
   Q61510           ESTROGEN-RESPONSIVE FINGER PROTEIN - MUS MUSCULUS (MOUSE).
   O70583           MIDLINE 1 (MIDLINE 1 PROTEIN) - MUS MUSCULUS (MOUSE).
   O35418           MIDLINE 1 (RING FINGER PROTEIN) - MUS MUSCULUS (MOUSE).
   O15344           PUTATIVE TRANSCRIPTION FACTOR XPRF (MIDLINE 1 FETAL KIDNEY I
   Q91453           STONUSTOXIN BETA-SUBUNIT - SYNANCEIA HORRIDA.

SCAN HISTORY SPTR37_10f 5 75 NSINGLE INITIAL MOTIF SETS BUTYPHLNCDUF1 Length of motif = 18 Motif number = 1 Butyrophylin C-terminal DUF motif I - 1 PCODE ST INT GLAPLTLDPNTAHPNLVL A33_PLEWA 444 444 HEVDVTLDPDTAHPHLFL BUTY_MOUSE 302 302 HAVDVTLDPDTAHPHLFL BUTY_HUMAN 301 301 HAVDVTLDPDTAHPHLFL BUTY_BOVIN 301 301 CAVHITLDPDTANPWLIL RO52_HUMAN 285 285 YSVDVTLDPDTAYPSLIL RFP_HUMAN 314 314 YSVDVTLDPDTAYPSLIL RFP_MOUSE 323 323 BUTYPHLNCDUF2 Length of motif = 18 Motif number = 2 Butyrophylin C-terminal DUF motif II - 1 PCODE ST INT LSEGLTSVKYTDTKQQLP A33_PLEWA 461 -1 LYEDSKSVRLEDSRQILP BUTY_MOUSE 319 -1 LYEDSKSVRLEDSRQKLP BUTY_HUMAN 318 -1 LYEDSKSVRLEDSRQKLP BUTY_BOVIN 318 -1 LSEDRRQVRLGDTQQSIP RO52_HUMAN 302 -1 LSDNLRQVRYSYLQQDLP RFP_HUMAN 331 -1 LSDNLRQVRYSYLQQDLP RFP_MOUSE 340 -1 BUTYPHLNCDUF3 Length of motif = 25 Motif number = 3 Butyrophylin C-terminal DUF motif III - 1 PCODE ST INT RFSQCILVLGAEGFDSGKHYWEVEV A33_PLEWA 483 4 RFDSWPCVLGRETFTSGRHYWEVEV BUTY_MOUSE 341 4 RFDSWPCVLGRETFTSGRHYWEVEV BUTY_HUMAN 340 4 RFDSWPCVMGREAFTSGRHYWEVEV BUTY_BOVIN 340 4 RFDSYPMVLGAQHFHSGKHYWEVDV RO52_HUMAN 324 4 RFNLFPCVLGSPCFIAGRHYWEVEV RFP_HUMAN 353 4 RFNLFPCVLGSPCFIAGRHYWEVEV RFP_MOUSE 362 4 BUTYPHLNCDUF4 Length of motif = 14 Motif number = 4 Butyrophylin C-terminal DUF motif IV - 1 PCODE ST INT WDVGMASESSNRKG A33_PLEWA 513 5 WAIGVCRENVVKKG BUTY_MOUSE 371 5 WAIGVCRENVMKKG BUTY_HUMAN 370 5 WAIGVCRENVMKKG BUTY_BOVIN 370 5 WDLGVCRDSVRRKG RO52_HUMAN 354 5 WTIGVCEDSVCRKG RFP_HUMAN 383 5 WTIGVCEDSVCRKG RFP_MOUSE 392 5 BUTYPHLNCDUF5 Length of motif = 25 Motif number = 5 Butyrophylin C-terminal DUF motif V - 1 PCODE ST INT LNLTSKPSKIGVYLDYEGGQVSFYN A33_PLEWA 557 30 LRLAGPPRRVGVFLDYDAGDISFYN BUTY_MOUSE 414 29 LPLAGPPRRVGIFLDYESGDISFYN BUTY_HUMAN 413 29 LPLAGPPRRVGVFLDYESGDIFFYN BUTY_BOVIN 413 29 LHLQVPPCQVGIFLDYEAGMVSFYN RO52_HUMAN 398 30 LPLRTPLQRVGIFLDYDAGEVSFYN RFP_HUMAN 427 30 LPLRTPLQRVGIFLDYDAGEVSFYN RFP_MOUSE 436 30 BUTYPHLNCDUF6 Length of motif = 19 Motif number = 6 Butyrophylin C-terminal DUF motif VI - 1 PCODE ST INT PIYTFNGSFTEKLYPYLSP A33_PLEWA 587 5 IYTFPSISFSGPLRPFFCL BUTY_MOUSE 445 6 IYTFSNVTFSGPLRPFFCL BUTY_HUMAN 444 6 IYTFSKASFSGPLRPFFCL BUTY_BOVIN 444 6 IYSFSECAFTGPLRPFFSP RO52_HUMAN 430 7 TFTFSHATFCGPVRPYFSL RFP_HUMAN 458 6 TFTFSHATFCGPVRPYFSL RFP_MOUSE 467 6 FINAL MOTIF SETS BUTYPHLNCDUF1 Length of motif = 18 Motif number = 1 Butyrophylin C-terminal DUF motif I - 5 PCODE ST INT HAVDVTLDPDTAHPHLFL BUTY_HUMAN 301 301 HEVDVTLDPDTAHPHLFL BUTY_MOUSE 302 302 HAADVVLDPDTAHPELFL O00480 325 325 HAVDVTLDPDTAHPHLFL BUTY_BOVIN 301 301 HAVDVVLDPDTAHPDLFL O00475 326 326 KPADVILDPDTANAILLV O00478 338 338 CAVHITLDPDTANPWLIL RO52_HUMAN 285 285 KPADVILDPKTANPILLV O00481 338 338 KPADVILDPKTANPILLV O75657 320 320 HAVDVVLDPDTAHPDLFL P78408 312 312 YSVDVTLDPDTAYPSLIL RFP_HUMAN 314 314 YSVDVTLDPDTAYPSLIL RFP_MOUSE 323 323 SAADVILYPDMANAILLV P78409 496 496 LTPDVILYPDMANAILLV O75658 311 311 HAANVVLDQDTGHPYLFV O75656 243 243 HQVSVTLDPDTAHHELIL O00635 290 290 HAVNVILDAETAYPNLIF O15553 596 596 FQVDMTLDADTANNFLLI O75677 94 94 CWVHITLDRNTANSWLII RO52_MOUSE 289 289 FQVDMTLDADTANNFLLI O75679 94 94 FQVDMTLDANTANNFLLI O75678 94 94 GLAPLTLDPNTAHPNLVL A33_PLEWA 444 444 SLTPMLLDPNSAHPNLHL Q91431 431 431 SLTPMLLDPTSAHPNLHL Q92021 430 430 DSGVITLDPQTASRSLVL O95604 292 292 KTVSVTLDPQSASGYLQL Q12899 311 311 BUTYPHLNCDUF2 Length of motif = 18 Motif number = 2 Butyrophylin C-terminal DUF motif II - 5 PCODE ST INT LYEDSKSVRLEDSRQKLP BUTY_HUMAN 318 -1 LYEDSKSVRLEDSRQILP BUTY_MOUSE 319 -1 LSEDRRSVRRGPYRQRVP O00480 342 -1 LYEDSKSVRLEDSRQKLP BUTY_BOVIN 318 -1 LSEDRRSVRRCPFRHLGE O00475 343 -1 VSEDQRSVQRAEEPRDLP O00478 355 -1 LSEDRRQVRLGDTQQSIP RO52_HUMAN 302 -1 VSEDQRSVQRAKEPQDLP O00481 355 -1 VSEDQRSVQRAKEPQDLP O75657 337 -1 LSEDRRSVRRCPFRHLGE P78408 329 -1 LSDNLRQVRYSYLQQDLP RFP_HUMAN 331 -1 LSDNLRQVRYSYLQQDLP RFP_MOUSE 340 -1 VSEDQRSVQRAEEPHDLP P78409 513 -1 VSEDQRSVQRAEEPHDLP O75658 328 -1 VSEDKRSVTLDPSRESIP O75656 260 -1 LSEDRRQVTRGYTQENQD O00635 307 -1 FSDDLKSVRLGNKWERLP O15553 613 -1 ISDDLRSVRSGCITQNRQ O75677 111 -1 ISKDRRQVRMGDTHQNVS RO52_MOUSE 306 -1 ISDDLRSVRSGLITQNRQ O75679 111 -1 ISDDLRSVRSGRIRQNRQ O75678 111 -1 LSEGLTSVKYTDTKQQLP A33_PLEWA 461 -1 LSDGLTSVRYGENKLSLP Q91431 448 -1 LSDGLTSVRYGENKLSLP Q92021 447 -1 LSEDRKSVRYTRQKKSLP O95604 309 -1 LSEDWKCVTYTSLYKSAY Q12899 328 -1 BUTYPHLNCDUF3 Length of motif = 25 Motif number = 3 Butyrophylin C-terminal DUF motif III - 5 PCODE ST INT RFDSWPCVLGRETFTSGRHYWEVEV BUTY_HUMAN 340 4 RFDSWPCVLGRETFTSGRHYWEVEV BUTY_MOUSE 341 4 RFDSQPCVLGWESFASGKHYWEVEV O00480 364 4 RFDSWPCVMGREAFTSGRHYWEVEV BUTY_BOVIN 340 4 RFDSQPCVLGRESFASGKHYWEVEV O00475 368 7 RFEWRYCVLGCENFTSGRHYWEVEV O00478 377 4 RFDSYPMVLGAQHFHSGKHYWEVDV RO52_HUMAN 324 4 RFNWHYCVLGCESFISGRHYWEVEV O00481 377 4 RFNWHYCVLGCESFISGRHYWEVEV O75657 359 4 RFDSQPCVLGRESFASGKHYWEVEV P78408 354 7 RFNLFPCVLGSPCFIAGRHYWEVEV RFP_HUMAN 353 4 RFNLFPCVLGSPCFIAGRHYWEVEV RFP_MOUSE 362 4 RFEWRYCVLGCESFMSERHYWEVEV P78409 535 4 RFEWRYCVLGCESFMSERHYWEVEV O75658 350 4 RFDSQLCVLGQESFASGKHYLEVDV O75656 282 4 RFTAFPCVLGCEGFTSGRRYFEVDV O00635 329 4 RFDSCIIVLGSPSFLSGRRYWEVEV O15553 635 4 RFDVSICILGSPRFTCGRHYWEVDV O75677 133 4 RFSNYPMVLGAQRFSSGKMYWEVDV RO52_MOUSE 328 4 RFDVSVCILGSPRFTCGRHYWEVDV O75679 133 4 RFDVSVCILGSPRFTCGRHCWEVDV O75678 133 4 RFSQCILVLGAEGFDSGKHYWEVEV A33_PLEWA 483 4 RFSQCILVLGSQGFDSGRHYWEVEV Q91431 470 4 RFSQCILVLGSQGFDSGRHYWEVEV Q92021 469 4 RFDGLPAVLGFPGFSSGRHRWQVDL O95604 331 4 QFDCEPGVLGSKGFTWGKVYWEVEV Q12899 350 4 BUTYPHLNCDUF4 Length of motif = 14 Motif number = 4 Butyrophylin C-terminal DUF motif IV - 5 PCODE ST INT WAIGVCRENVMKKG BUTY_HUMAN 370 5 WAIGVCRENVVKKG BUTY_MOUSE 371 5 WTVGVCRHSVERKG O00480 394 5 WAIGVCRENVMKKG BUTY_BOVIN 370 5 WTVGVCRDSVERKG O00475 398 5 WHIGVCSKNVERKK O00478 407 5 WDLGVCRDSVRRKG RO52_HUMAN 354 5 WHIGVCSKNVQRKG O00481 407 5 WHIGVCSKNVQRKG O75657 389 5 WTVGVCRDSVERKG P78408 384 5 WTIGVCEDSVCRKG RFP_HUMAN 383 5 WTIGVCEDSVCRKG RFP_MOUSE 392 5 WHIGVCSKNVERKK P78409 565 5 WHIGVCSKNVERKK O75658 380 5 WTVGICRDNVERKW O75656 312 5 WDLGVCMENVQRGT O00635 359 5 WILGACKTSISRKG O15553 665 5 WDLGVCRESVHRKG O75677 163 5 WDLGVCRDSVQRKG RO52_MOUSE 358 5 WDLGVCRESVHCKG O75679 163 5 WDLGVCRESVHRKG O75678 163 5 WDVGMASESSNRKG A33_PLEWA 513 5 WDVGMASESSNRKG Q91431 500 5 WDVGMASESSNRKG Q92021 499 5 CTVGVAGEGVRRKG O95604 363 7 CMVGVARDSVKRKG Q12899 437 62 BUTYPHLNCDUF5 Length of motif = 25 Motif number = 5 Butyrophylin C-terminal DUF motif V - 5 PCODE ST INT LPLAGPPRRVGIFLDYESGDISFYN BUTY_HUMAN 413 29 LRLAGPPRRVGVFLDYDAGDISFYN BUTY_MOUSE 414 29 LPLKESLCRVGVFLDYEAGDVSFYN O00480 437 29 LPLAGPPRRVGVFLDYESGDIFFYN BUTY_BOVIN 413 29 LPLKESLCRVGVFLDYEAGDVSFYN O00475 441 29 LKLPEPPRKVGIFLDYETGEISFYN O00478 452 31 LHLQVPPCQVGIFLDYEAGMVSFYN RO52_HUMAN 398 30 LKLPKPPKKVGVFLDYETGDISFYN O00481 451 30 LKLPKPPKKVGVFLDYETGDISFYN O75657 433 30 LPLKESLCRVGVFLDYEAGDVSFYN P78408 427 29 LPLRTPLQRVGIFLDYDAGEVSFYN RFP_HUMAN 427 30 LPLRTPLQRVGIFLDYDAGEVSFYN RFP_MOUSE 436 30 LKLPEPPRKVGVILDYETGHISFYN P78409 610 31 LKLPEPPRKVGVILDYETGHISFYN O75658 425 31 LSLEEPLCQVGIFLDYEAGDVSFYN O75656 355 29 LHLHEQPLLVGIFLDYEAGVVSFYN O00635 403 30 LLIKEPPKRVGIFVDYRVGSISFYN O15553 709 30 LFVDRKLQRVGIFLDMGMQNVSFFD O75677 207 30 LHIQVPPCQIGIFVDYEAGVVSFYN RO52_MOUSE 401 29 LLVDRKLQRVGIFLDMGMQNVSFFD O75679 207 30 LFVDRKLQRVGIFLDMGMQNVSFFD O75678 207 30 LNLTSKPSKIGVYLDYEGGQVSFYN A33_PLEWA 557 30 LSLTSHPRKIGVYVDYEGGQISFYN Q91431 544 30 LSLSSHPRKIGVYVDYEGGQISFYN Q92021 543 30 LPLSEIPRGVRVALDYEAGQVTLHN O95604 406 29 LFPALRPRRVGIALDYEGGTVTFTN Q12899 480 29 BUTYPHLNCDUF6 Length of motif = 19 Motif number = 6 Butyrophylin C-terminal DUF motif VI - 5 PCODE ST INT IYTFSNVTFSGPLRPFFCL BUTY_HUMAN 444 6 IYTFPSISFSGPLRPFFCL BUTY_MOUSE 445 6 IYTCPRSAFTVPVRPFFRL O00480 468 6 IYTFSKASFSGPLRPFFCL BUTY_BOVIN 444 6 IYTCPRSAFSVPVRPFFRL O00475 472 6 IYTFPHASFSEPLYPVFRI O00478 483 6 IYSFSECAFTGPLRPFFSP RO52_HUMAN 430 7 IHTFLDVSFSEALYPVFRI O00481 482 6 IHTFLDVSFSEALYPVFRI O75657 464 6 IYTCPRSAFSGPDTSQSGD P78408 458 6 TFTFSHATFCGPVRPYFSL RFP_HUMAN 458 6 TFTFSHATFCGPVRPYFSL RFP_MOUSE 467 6 IYTFLHASSSEPLYPVFRI P78409 641 6 IYTFLHASSSEPLYPVFRI O75658 456 6 IYTFPHSAFSVPVRPFFSL O75656 386 6 IFTFPKASFSDTLRPYFQV O00635 434 6 IYTFASCSFSGPLQPIFSP O15553 740 6 VYTFRSVSAEEPLHLFFAP O75677 238 6 IYTFSECVFAGPLRPFFNV RO52_MOUSE 433 7 VYTFRSVSAEEPLRPFLAP O75679 238 6 VYTFRSVSAEEPLCPFLAP O75678 238 6 PIYTFNGSFTEKLYPYLSP A33_PLEWA 587 5 IIYTFSATFTEKLYPYLSP Q91431 574 5 IIYTFNATFTEKLYPYLSP Q92021 573 5 PIFTFTASFSGKVFPFFAV O95604 436 5 LIYTFTATFTRRLVPFLWL Q12899 510 5

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