WORKLIST ENTRIES (1):
ARGDEIMINASE View alignment Bacterial arginine deiminase signature
Type of fingerprint: COMPOUND with 6 elements
Links:
PRINTS; PR00102 OTCASE
PFAM; PF02726 Arg_deiminase
INTERPRO; IPR003876
Creation date 08-JAN-2001
1. BROWN, D.M., UPCROFT, J.A., EDWARDS, M.R. AND UPCROFT, P.
Anaerobic bacterial metabolism in the ancient eukaryote Giardia duodenalis.
INT.J.PARASITOL. 28 149-64 (1998).
2. HARASAWA, R., KOSHIMIZU, K., KITAGAWA, M., ASADA, K. AND KATO, I.
Nucleotide sequence of the arginine deiminase gene of Mycoplasma hominis.
MICROBIOL.IMMUNOL. 36 661-665 (1992).
3. KANAOKA, M., KAWANAKA, C., NEGORO, T., FUKITA, Y., TAYA, K. AND AGUI, H.
Cloning and expression of the antitumor glycoprotein gene of Streptococcus
pyogenes Su in Escherichia coli.
AGRIC.BIOL.CHEM. 51 2641-2648 (1987).
4. DEGNAN, B.A., PALMER, J.M., ROBSON, T., JONES, C.E., FISCHER, M.,
GLANVILLE, M., MELLOR, G.D., DIAMOND, A.G., KEHOE, M.A. AND GOODACRE, J.A.
Inhibition of human peripheral blood mononuclear cell proliferation by
Streptococcus pyogenes cell extract is associated with arginine deiminase
activity.
INFECT.IMMUN. 66 3050-3058 (1998).
The arginine dihydrolase (AD) pathway is found in many prokaryotes and some
primitive eukaryotes, an example of the latter being Giardia [1}. The three-
enzyme anaerobic pathway breaks down L-arginine to form 1 mol of ATP, carbon
dioxide and ammonia. In simpler bacteria, the first enzyme, arginine
deiminase, can account for up to 10% of total cell protein [1].
Arginine deiminase catalyses the conversion of L-arginine to L-citrulline
and ammonia. As well as producing energy via ATP, the ammonia also serves
to protect the bacteria against acid damage, and the citrulline generated
can be used in other biosynthetic pathways [2]. A streptococcal acid
glycoprotein (SAGP) has also been shown to function as an arginine
deiminase [3].
Recently, another function of this enzyme has been discovered [4]. It has a
potent anti-tumour effect, and can inhibit antigen, superantigen, or mitogen
-stimulated human peripheral blood mononuclear cell proliferation [4].
Another function of the protein may be to inhibit cell proliferation by
cell cycle arrest and apoptosis induction. It has thus been hypothesised
that recombinant arginine deiminase could be used as a novel anti-tumour
agent [4].
ARGDEIMINASE is a 6-element fingerprint that provides a signature for
the bacterial arginine deiminase protein family. The fingerprint was
derived from an initial alignment of 4 sequences: the motifs were drawn from
conserved regions spanning the full alignment length (~430 amino acids). Two
iterations on SPTR37_10f were required to reach convergence, at which point
a true set comprising 13 sequences was identified. Three partial matches
were also found: P75475 and P75474 are Mycoplasma pneumoniae arginine
deiminases that match the first three and the last three motifs respectively;
and Q48294 is a Halobacterium salinarium arginine deiminase that matches
motifs 2 and 6.
SUMMARY INFORMATION
13 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
2 codes involving 3 elements
1 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
6| 13 13 13 13 13 13
5| 0 0 0 0 0 0
4| 0 0 0 0 0 0
3| 1 1 1 1 1 1
2| 0 1 0 0 0 1
--+-------------------------------
| 1 2 3 4 5 6
True positives..
O51896 Q46254 SAGP_STRPY O51781
O86131 O53088 ARCA_PSEAE ARCA_MYCHO
O31017 ARCA_MYCAR ARCA_PSEPU O05585
ARCA_MYCPN
Subfamily: Codes involving 3 elements
Subfamily True positives..
P75475 P75474
Subfamily: Codes involving 2 elements
Subfamily True positives..
Q48294
PROTEIN TITLES
O51896 ARCA - BORRELIA AFZELII.
Q46254 ARGININE DEIMINASE - CLOSTRIDIUM PERFRINGENS.
SAGP_STRPY STREPTOCOCCAL ACID GLYCOPROTEIN - STREPTOCOCCUS PYOGENES.
O51781 ARGININE DEIMINASE (ARCA) - BORRELIA BURGDORFERI (LYME DISEA
O86131 ARGININE DEIMINASE (EC 3.5.3.6) (ARGININE DIHYDROLASE) - BAC
O53088 ARGININE DEIMINASE (EC 3.5.3.6) (ARGININE DIHYDROLASE) - LAC
ARCA_PSEAE ARGININE DEIMINASE (EC 3.5.3.6) - PSEUDOMONAS AERUGINOSA.
ARCA_MYCHO ARGININE DEIMINASE (EC 3.5.3.6) - MYCOPLASMA HOMINIS.
O31017 ARCA (EC 3.5.3.6) (ARGININE DEIMINASE) (ARGININE DIHYDROLASE
ARCA_MYCAR ARGININE DEIMINASE (EC 3.5.3.6) - MYCOPLASMA ARGININI.
ARCA_PSEPU ARGININE DEIMINASE (EC 3.5.3.6) - PSEUDOMONAS PUTIDA.
O05585 ARCA - MYCOBACTERIUM TUBERCULOSIS.
ARCA_MYCPN ARGININE DEIMINASE (EC 3.5.3.6) - MYCOPLASMA PNEUMONIAE.
P75475 ARGININE DEIMINASE - MYCOPLASMA PNEUMONIAE.
P75474 ARGININE DEIMINASE - MYCOPLASMA PNEUMONIAE.
Q48294 ARGININE DESAMINASE - HALOBACTERIUM SALINARIUM.
SCAN HISTORY
SPTR37_10f 2 75 NSINGLE
INITIAL MOTIF SETS
ARGDEIMINASE1 Length of motif = 21 Motif number = 1
Bacterial arginine deiminase motif I - 1
PCODE ST INT
TPIHVYSEIGKLKKVLLHRPG SAGP_STRPY 4 4
NGIHVYSEIGELETVLVHEPG ARCA_MYCHO 8 8
TKLGVHSEAGKLRKVMVCSPG ARCA_PSEAE 5 5
SGVRVTSEISFLREVIAHHPG ARCA_MYCPN 32 32
ARGDEIMINASE2 Length of motif = 19 Motif number = 2
Bacterial arginine deiminase motif II - 1
PCODE ST INT
PMPNLYFTRDPFATIGTGV SAGP_STRPY 156 131
PMPNLYFTRDPFASVGNGV ARCA_MYCHO 150 121
PLPNTQFTRDTTCWIYGGV ARCA_PSEAE 156 130
PLSFSLYPHKFFNTLGTGV ARCA_MYCPN 180 127
ARGDEIMINASE3 Length of motif = 16 Motif number = 3
Bacterial arginine deiminase motif III - 1
PCODE ST INT
RNRETLYGKYIFTHHP SAGP_STRPY 184 9
RRRETLFARFVFRNHP ARCA_MYCHO 178 9
RRQETLLTTAIYKFHP ARCA_PSEAE 184 9
LKRHSLVYEYIMRFHP ARCA_MYCPN 208 9
ARGDEIMINASE4 Length of motif = 18 Motif number = 4
Bacterial arginine deiminase motif IV - 1
PCODE ST INT
REQWNDGSNTLTIAPGVV SAGP_STRPY 346 146
RETNFDGTNYLAIKPGLV ARCA_MYCHO 342 148
REQWDDGNNVVCLEPGVV ARCA_PSEAE 351 151
KELLDCGMGVLNLSPGEI ARCA_MYCPN 376 152
ARGDEIMINASE5 Length of motif = 15 Motif number = 5
Bacterial arginine deiminase motif V - 1
PCODE ST INT
YNRNTITNAILESKG SAGP_STRPY 366 2
YDRNEKTNAALKAAG ARCA_MYCHO 362 2
YDRNTYTNTLLRKAG ARCA_PSEAE 371 2
FDRNHYTNNLLNELG ARCA_MYCPN 396 2
ARGDEIMINASE6 Length of motif = 19 Motif number = 6
Bacterial arginine deiminase motif VI - 1
PCODE ST INT
SELVRGRGGPRCMSMPFER SAGP_STRPY 389 8
NQLSLGMGNARCMSMPLSR ARCA_MYCHO 385 8
SELGRGRGGGHCMTCPIVR ARCA_PSEAE 394 8
SELSRGPSGPLEMVCSLWR ARCA_MYCPN 419 8
FINAL MOTIF SETS
ARGDEIMINASE1 Length of motif = 21 Motif number = 1
Bacterial arginine deiminase motif I - 2
PCODE ST INT
NPINIFSEIGRLKKVLLHRPG O51896 6 6
RALNVTSEIGRLKTVLLHRPG Q46254 5 5
TPIHVYSEIGKLKKVLLHRPG SAGP_STRPY 4 4
NPINIFSEIGRLKKVLLHRPG O51781 7 7
TPIHVYSEIGPLKTVMLKRPG O86131 5 5
SPIHVNSEIGKLKTVLLKRPG O53088 3 3
TKLGVHSEAGKLRKVMVCSPG ARCA_PSEAE 5 5
NGIHVYSEIGELETVLVHEPG ARCA_MYCHO 8 8
RGFGVHSEVGKLRTVMVCRPS O31017 2 2
KGIHVYSEIGELESVLVHEPG ARCA_MYCAR 8 8
QKYGVHSEAGKLRKVMVCSPG ARCA_PSEPU 6 6
VELGSNSEVGALRVVILHRPG O05585 3 3
SGVRVTSEISFLREVIAHHPG ARCA_MYCPN 32 32
ARGDEIMINASE2 Length of motif = 19 Motif number = 2
Bacterial arginine deiminase motif II - 2
PCODE ST INT
PMPNVLFTRDPFASIGNGV O51896 155 128
PMPNLYFTREPFATIGHGI Q46254 156 130
PMPNLYFTRDPFATIGTGV SAGP_STRPY 156 131
PMPNVLFTRDPFASIGNGI O51781 156 128
PMPNLYFTRDPAAAIGSGL O86131 154 128
PMPNLYFTRDPAASMGDGL O53088 152 128
PLPNTQFTRDTTCWIYGGV ARCA_PSEAE 156 130
PMPNLYFTRDPFASVGNGV ARCA_MYCHO 150 121
PIPNTLFQRDPSCWIYGGV O31017 150 127
PMPNLYFTRDPFASVGNGV ARCA_MYCAR 151 122
PLPNTQFTRDTTCWIYGGV ARCA_PSEPU 157 130
PLPNLVFTRDSSIWIGPRV O05585 154 130
PLSFSLYPHKFFNTLGTGV ARCA_MYCPN 180 127
ARGDEIMINASE3 Length of motif = 16 Motif number = 3
Bacterial arginine deiminase motif III - 2
PCODE ST INT
RQRETIFAEYIFKYHP O51896 183 9
RNRETIFAKYIFRHHP Q46254 184 9
RNRETLYGKYIFTHHP SAGP_STRPY 184 9
RRRETIFAEYIFKYHS O51781 184 9
RRRESLFMRYIINHHP O86131 182 9
RQRESMFMEVIMQHHP O53088 180 9
RRQETLLTTAIYKFHP ARCA_PSEAE 184 9
RRRETLFARFVFRNHP ARCA_MYCHO 178 9
RRAETLIQRAIYKFHP O31017 178 9
RQRETLFSRFVFSNHP ARCA_MYCAR 179 9
RRQETLLTTAIYKFHK ARCA_PSEPU 185 9
RVREASLTDLIYAHHP O05585 182 9
LKRHSLVYEYIMRFHP ARCA_MYCPN 208 9
ARGDEIMINASE4 Length of motif = 18 Motif number = 4
Bacterial arginine deiminase motif IV - 2
PCODE ST INT
REQWNDGANVLAIAPGEI O51896 345 146
REQWNDGSNTLAIAPGEV Q46254 349 149
REQWNDGSNTLTIAPGVV SAGP_STRPY 346 146
REQWNDGANVLAIAPGEV O51781 346 146
REQWNDGSNTLAIAPGVV O86131 348 150
REQWNDGSNTLAIAPGVV O53088 345 149
REQWDDGNNVVCLEPGVV ARCA_PSEAE 351 151
RETNFDGTNYLAIKPGLV ARCA_MYCHO 342 148
REQWDDGNNVVALEPGVV O31017 344 150
RETHFDGTNYLAIRPGVV ARCA_MYCAR 343 148
REQWDDGNNVVALEPGVV ARCA_PSEPU 354 153
REQWDDGNNTLALAPGVV O05585 338 140
KELLDCGMGVLNLSPGEI ARCA_MYCPN 376 152
ARGDEIMINASE5 Length of motif = 15 Motif number = 5
Bacterial arginine deiminase motif V - 2
PCODE ST INT
YSRNHVTNKLFEENG O51896 365 2
YSRNYVTNEILEKEG Q46254 369 2
YNRNTITNAILESKG SAGP_STRPY 366 2
YSRNHVTNKLFEENG O51781 366 2
YDRNYISNECLREQG O86131 368 2
YDRNYVSNENLRQYG O53088 365 2
YDRNTYTNTLLRKAG ARCA_PSEAE 371 2
YDRNEKTNAALKAAG ARCA_MYCHO 362 2
YDRNTYTNTLLRKAG O31017 364 2
YSRNEKTNAALEAAG ARCA_MYCAR 363 2
YDRNTYTNTLLRKAG ARCA_PSEPU 374 2
YERNVQTNARLQDAG O05585 358 2
FDRNHYTNNLLNELG ARCA_MYCPN 396 2
ARGDEIMINASE6 Length of motif = 19 Motif number = 6
Bacterial arginine deiminase motif VI - 2
PCODE ST INT
SELSRGRGGPRCMSMPLIR O51896 388 8
SELSRGRGGPRCMSMPLIR Q46254 392 8
SELVRGRGGPRCMSMPFER SAGP_STRPY 389 8
SELSRGRGGPRCMSMSLVR O51781 389 8
GELSRGRGGPRCMSMPLYR O86131 391 8
SELSRGRGGPRCMSMPLVR O53088 388 8
SELGRGRGGGHCMTCPIVR ARCA_PSEAE 394 8
NQLSLGMGNARCMSMPLSR ARCA_MYCHO 385 8
SELGRGRGGGHCMTCPIWR O31017 387 8
NQLSLGMGNARCMSMPLSR ARCA_MYCAR 386 8
GELGRGRGGGHCMTCPIVR ARCA_PSEPU 397 8
SELGTGRGGPRCMSCPAAR O05585 381 8
SELSRGPSGPLEMVCSLWR ARCA_MYCPN 419 8
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