WORKLIST ENTRIES (1):
ANIONEXHNGR1 View alignment Anion exchanger isoform 1 signature
Type of fingerprint: COMPOUND with 4 elements
Links:
PRINTS; PR00165 ANIONEXCHNGR; PR01188 ANIONEXHNGR2; PR01189 ANIONEXHNGR3
INTERPRO; IPR002977
Creation date 15-JUN-1999
1. KOPITO, R.R.
Molecular biology of the anion exchanger gene family.
INT.REV.CYTOL. 123 177-199 (1990).
2. ALPER, S.L.
The band 3-related anion exchanger (AE) gene family.
ANNU.REV.PHYSIOL. 53 549-564 (1991).
3. TANNER, M.J.
The structure and function of band 3 (AE1): recent developments (review).
MOL.MEMBR.BIOL. 14 155-165 (1997).
4. BRUCE, L.J., UNWIN, R.J., WRONG, O. AND TANNER, M.J.
The association between familial distal renal tubular acidosis and
mutations in the red cell anion exchanger (band 3, AE1) gene.
BIOCHEM.CELL BIOL. 76 723-728 (1998).
Anion exchange proteins are thought to participate in pH and cell volume
regulation. They are glycosylated, plasma-membrane transport proteins that
exchange hydrogen carbonate (HCO3-) for chloride (Cl-) in a reversible,
electroneutral manner [1,2]. To date three anion exchanger isoforms have
been identified (AE1-3), AE1 being the previously-characterised erythrocyte
band 3 protein. They share a predicted topology of 12-14 transmembrane (TM)
domains, but have differing distribution patterns and cellular localisation.
The best characterised isoform, AE1, is known to be the most abundant
membrane protein in mature erythrocytes. It has a molecular mass of ~95 kDa
and consists of two major domains. The N-terminal 390 residues form a water-
soluble, highly elongated domain that serves as an attachment site for the
binding of the membrane skeleton and other cytoplasmic proteins. The
remainder of the protein is a 55kDa hydrophobic domain that is responsible
for catalysing anion exchange. The function of the analogous domains of AE2
and AE3 remains to be determined [3].
Naturally-occuring mutations have been characterised in the AE1 gene, which
give rise to forms of several inherited human diseases. Around 20% of
hereditary spherocytosis cases arise from heterozygosity for AE1 mutations,
and result in the absence or decrease of the mutant protein in the red cell
membrane. Similarly, familial distal renal tubular acidosis, a condition
associated with kidney stones, has been shown to be associated with
mutations of AE1 of the renal collecting duct alpha-intercalated cell, and
it has been postulated that such mutations may affect the targeting of the
AE1 protein, which is usually directed to the basolateral membrane of
these cells [4].
ANIONEXHNGR1 is a 4-element fingerprint that provides a signature for the
anion exchanger isoform 1. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections
that characterise the anion exchanger isoform 1 but distinguish it from
others - motif 1 resides within the putative cytoplasmic N-terminus; motif
2 lies on the exoplasmic loop connecting putative TM domains 7 and 8; motif
3 encodes a small portion of the intracellular loop between TM domains 8
and 9; and motif 4 is located on the putative extracellular loop connecting
TM domains 11 and 12. Two iterations on SPTR37_9f were required to reach
convergence, at which point a true set comprising 7 sequences was identified.
SUMMARY INFORMATION
7 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
4| 7 7 7 7
3| 0 0 0 0
2| 0 0 0 0
--+---------------------
| 1 2 3 4
True positives..
B3AT_HUMAN B3AT_RAT B3AT_MOUSE B3AT_CHICK
Q90579 B3AT_ONCMY Q91452
PROTEIN TITLES
B3AT_HUMAN BAND 3 ANION TRANSPORT PROTEIN (ANION EXCHANGE PROTEIN 1) (A
B3AT_RAT BAND 3 ANION EXCHANGE PROTEIN, KIDNEY - RATTUS NORVEGICUS (R
B3AT_MOUSE BAND 3 ANION EXCHANGE PROTEIN (MEB3) - MUS MUSCULUS (MOUSE).
B3AT_CHICK BAND 3 ANION TRANSPORT PROTEIN - GALLUS GALLUS (CHICKEN).
Q90579 ERYTHROID ANION TRANSPORTER - GALLUS GALLUS (CHICKEN).
B3AT_ONCMY BAND 3 ANION EXCHANGE PROTEIN - ONCORHYNCHUS MYKISS (RAINBOW
Q91452 ANION EXCHANGER - ONCORHYNCHUS MYKISS (RAINBOW TROUT) (SALMO
SCAN HISTORY
SPTR37_9f 2 150 NSINGLE
INITIAL MOTIF SETS
ANIONEXHNGR11 Length of motif = 10 Motif number = 1
Anion exchanger isoform 1 motif I - 1
PCODE ST INT
TDAPSEQALL B3AT_HUMAN 324 324
QEVPSEQHLH B3AT_CHICK 331 331
TEIQDEGMLQ B3AT_ONCMY 311 311
TDAPSEKALL B3AT_MOUSE 337 337
TEAPSEKALL B3AT_RAT 337 337
ANIONEXHNGR12 Length of motif = 9 Motif number = 2
Anion exchanger isoform 1 motif II - 1
PCODE ST INT
GFKVSNSSA B3AT_HUMAN 637 303
GLEVTNGTA B3AT_CHICK 648 307
GLMVSNPNA B3AT_ONCMY 650 329
GLKVSNSSA B3AT_MOUSE 655 308
GLKVSNSSA B3AT_RAT 653 306
ANIONEXHNGR13 Length of motif = 6 Motif number = 3
Anion exchanger isoform 1 motif III - 1
PCODE ST INT
PERKMV B3AT_HUMAN 692 46
PERKLV B3AT_CHICK 703 46
PERKMV B3AT_ONCMY 705 46
PERKMI B3AT_MOUSE 710 46
PERKMI B3AT_RAT 708 46
ANIONEXHNGR14 Length of motif = 7 Motif number = 4
Anion exchanger isoform 1 motif IV - 1
PCODE ST INT
YHPDVPY B3AT_HUMAN 818 120
YHPKEPY B3AT_CHICK 829 120
YYPADAY B3AT_ONCMY 827 116
YHPDVPF B3AT_MOUSE 836 120
YHPDVPF B3AT_RAT 834 120
FINAL MOTIF SETS
ANIONEXHNGR11 Length of motif = 10 Motif number = 1
Anion exchanger isoform 1 motif I - 2
PCODE ST INT
TDAPSEQALL B3AT_HUMAN 324 324
TEAPSEKALL B3AT_RAT 337 337
TDAPSEKALL B3AT_MOUSE 337 337
QEVPSEQHLH B3AT_CHICK 331 331
QEVPSEQHLH Q90579 253 253
TEIQDEGMLQ B3AT_ONCMY 311 311
TEIQDKGMLQ Q91452 305 305
ANIONEXHNGR12 Length of motif = 9 Motif number = 2
Anion exchanger isoform 1 motif II - 2
PCODE ST INT
GFKVSNSSA B3AT_HUMAN 637 303
GLKVSNSSA B3AT_RAT 653 306
GLKVSNSSA B3AT_MOUSE 655 308
GLEVTNGTA B3AT_CHICK 648 307
GLEVTNGTA Q90579 570 307
GLMVSNPNA B3AT_ONCMY 650 329
GLMVSNPNA Q91452 644 329
ANIONEXHNGR13 Length of motif = 6 Motif number = 3
Anion exchanger isoform 1 motif III - 2
PCODE ST INT
PERKMV B3AT_HUMAN 692 46
PERKMI B3AT_RAT 708 46
PERKMI B3AT_MOUSE 710 46
PERKLV B3AT_CHICK 703 46
PERKLV Q90579 625 46
PERKMV B3AT_ONCMY 705 46
PERKMV Q91452 699 46
ANIONEXHNGR14 Length of motif = 7 Motif number = 4
Anion exchanger isoform 1 motif IV - 2
PCODE ST INT
YHPDVPY B3AT_HUMAN 818 120
YHPDVPF B3AT_RAT 834 120
YHPDVPF B3AT_MOUSE 836 120
YHPKEPY B3AT_CHICK 829 120
YHPKEPY Q90579 751 120
YYPADAY B3AT_ONCMY 827 116
YYPRDAY Q91452 821 116
User query: Display/Full Code "ANIONEXHNGR1"