WORKLIST ENTRIES (1):
ADRENRGCA2AR View alignment View Structure Alpha-2A adrenergic receptor signature
Type of fingerprint: COMPOUND with 7 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR01103 ADRENERGICR; PR00559 ADRENRGCA2BR; PR00560 ADRENRGCA2CR
INTERPRO; IPR001946
Creation date 15-AUG-1996; UPDATE 06-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
Adrenaline and noradrenaline.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.32-54.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
In the periphery, the adrenergic system plays an important role in
regulating the cardiovascular system [6]. Increased sympathetic discharge
to the heart increases the rate and force of contraction mediated through
beta-1 receptors. Circulating adrenaline also acts on cardiac tissue, and,
in addition acts both on alpha-1 adrenoceptors in arterial smooth muscle,
stimulating vasoconstriction, and on beta-2 adrenoceptors in vascular beds
of skeletal muscle, stimulating vasodilation [6]. In the CNS, noradrenaline
is thought to be involved in the regulation of mood, and various psycho-
active drugs alter noradrenergic function. Numerous drugs exert their
actions via adrenoceptors: e.g., beta-2 selective agonists such as
salbutamol are used in the acute treatment of asthma, while alpha agonists
prolong the action of local anaesthetics, and act as nasal decongestants [6].
Adrenoceptors can be divided into three main classes based on sequence
similarity, receptor pharmacology and signalling mechanisms. Further
subdivisions exist within each class [6]. A large number of agonists and
antagonists distinguish between the different classes of adrenoceptor; by
contrast, relatively small differences in agonist and antagonist affinities
are demonstrated, especially within the alpha-1 and alpha-2 adrenoceptor
subtypes [6].
A wide distribution of alpha-2A receptor mRNA has been demonstrated by
Northern analysis, high levels occurring in rat CNS (e.g., hippocampus,
cerebral cortex, brainstem, pituitary gland and cerebellum), and in
peripheral tissues (e.g., kidney, aorta, skeletal muscle, spleen and lung)
[6]. The receptor inhibits adenylyl cyclase and L-type calcium channels,
and activates potassium channels through pertussis-toxin-insensitive
G proteins belonging to the Gi/G0 class [6].
ADRENRGCA2AR is a 7-element fingerprint that provides a signature for the
alpha-2A adrenergic receptors. The fingerprint was derived from an initial
alignment of 8 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the alpha-2A adrenergic receptors but
distinguish them from the rest of the rhodopsin-like superfamily - motif
1 lies at the N-terminus; motif 2 spans the second external loop; motifs
3-6 span the third cytoplasmic loop; and motif 7 lies at the C-terminus.
A single iteration on OWL28.1 was required to reach convergence, no
further sequences being identified beyond the starting set.
An update on SPTR37_9f identified a true set of 5 sequences.
SUMMARY INFORMATION
5 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
7| 5 5 5 5 5 5 5
6| 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0
--+------------------------------------
| 1 2 3 4 5 6 7
True positives..
A2AA_MOUSE A2AA_HUMAN A2AA_RAT A2AA_PIG
A2AA_CAVPO
PROTEIN TITLES
A2AA_MOUSE ALPHA-2A ADRENERGIC RECEPTOR (ALPHA-2A ADRENOCEPTOR) (ALPHA-
A2AA_HUMAN ALPHA-2A ADRENERGIC RECEPTOR (ALPHA-2A ADRENOCEPTOR) (ALPHA-
A2AA_RAT ALPHA-2A ADRENERGIC RECEPTOR (ALPHA-2A ADRENOCEPTOR) (ALPHA-
A2AA_PIG ALPHA-2A ADRENERGIC RECEPTOR (ALPHA-2A ADRENOCEPTOR) (ALPHA-
A2AA_CAVPO ALPHA-2A ADRENERGIC RECEPTOR (ALPHA-2A ADRENOCEPTOR) (ALPHA-
SCAN HISTORY
OWL28_1 1 50 NSINGLE
SPTR37_9f 2 6 NSINGLE
INITIAL MOTIF SETS
ADRENRGCA2AR1 Length of motif = 23 Motif number = 1
A2A adrenergic receptor motif I - 1
PCODE ST INT
PDAGNASWNGTEAPGGGARATPY A2AA_HUMAN 6 6
PDAGNSSWNGTEAPGGGTRATPY A2AA_MOUSE 6 6
PDAGNSSWNGTEAPGGGTRATPY A2AA_RAT 6 6
PDAGNASWNGTEAPGGGARATPY HUMADRA 6 6
PDAGNASWNGTEAPGGGARATPY HUMADRA2R 6 6
PDAGNSSWNGTEAPGGGTRATPY RATRG20 6 6
PEAGNASWNGTEAPGGGARATPY A2AA_PIG 6 6
PDSGNASWNGTEGPGGGTRATPY CPU25722 6 6
ADRENRGCA2AR2 Length of motif = 24 Motif number = 2
A2A adrenergic receptor motif II - 1
PCODE ST INT
SIEKKGGGGGPQPAEPRCEINDQK A2AA_HUMAN 171 142
SIEKKGAGGGQQPAEPSCKINDQK A2AA_MOUSE 171 142
SIEKKGAGGGQQPAEPSCKINDQK A2AA_RAT 171 142
SIEKKGGGGGPQPAEPRCEINDQK HUMADRA 171 142
SIEKKGGGGGPQPAEPRCEINDQK HUMADRA2R 171 142
SIEKKGAGGGQQPAEPSCKINDQK RATRG20 171 142
SIEKKAGGGGQQPAEPRCEINDQK A2AA_PIG 171 142
ISFEKAGGGGQQPAEPRCEINDQK CPU25722 170 141
ADRENRGCA2AR3 Length of motif = 20 Motif number = 3
A2A adrenergic receptor motif III - 1
PCODE ST INT
PSRRGPDAVAAPPGGTERRP A2AA_HUMAN 231 36
PSRRGPDACSAPPGGADRRP A2AA_MOUSE 231 36
PSRRGPDACSAPPGGADRRP A2AA_RAT 231 36
PSRRGPDAVAAPPGGTERRP HUMADRA 231 36
PSRRGPDAVAAPPGGTERRP HUMADRA2R 231 36
PSRRGPDACSAPPGGADRRP RATRG20 231 36
PSRRGPDAAAALPGGAERRP A2AA_PIG 231 36
PSRRGPDAHAAAPPGGAERR CPU25722 230 36
ADRENRGCA2AR4 Length of motif = 20 Motif number = 4
A2A adrenergic receptor motif IV - 1
PCODE ST INT
ERSAGPGGAEAEPLPTQLNG A2AA_HUMAN 256 5
ERGAGPTGAEAEPLPTQLNG A2AA_MOUSE 256 5
ERGAGTAGAEAEPLPTQLNG A2AA_RAT 256 5
ERSAGPGGAEAEPLPTQLNG HUMADRA 256 5
ERSAGPGGAEAEPLPTQLNG HUMADRA2R 256 5
ERGAGTAGAEAEPLPTQLNG RATRG20 256 5
ERGVGRVGAEAEPLPVQLNG A2AA_PIG 256 5
ERGVGPGGAEAEPLPTQVNG CPU25722 256 6
ADRENRGCA2AR5 Length of motif = 19 Motif number = 5
A2A adrenergic receptor motif V - 1
PCODE ST INT
EPAPAGPRDTDALDLEESS A2AA_HUMAN 279 3
EPAPAGPRDGDALDLEESS A2AA_MOUSE 279 3
EPAPTRPRDGDALDLEESS A2AA_RAT 279 3
EPAPAGPRDTDALDLEESS HUMADRA 279 3
EPAPAGPRDTDALDLEESS HUMADRA2R 279 3
EPAPTRPRDGDALDLEESS RATRG20 279 3
EPAPAGPRDADGLDLEESS A2AA_PIG 279 3
EPAPAGPRDAEALDLEESS CPU25722 279 3
ADRENRGCA2AR6 Length of motif = 18 Motif number = 6
A2A adrenergic receptor motif VI - 1
PCODE ST INT
RPERGPRGKGKARASQVK A2AA_HUMAN 310 12
RPDRGPRAKGKTRASQVK A2AA_MOUSE 310 12
KPERGPRAKGKTKASQVK A2AA_RAT 310 12
RPERGPRGKGKARASQVK HUMADRA 310 12
RPERGPRGKGKARASQVK HUMADRA2R 310 12
KPERGPRAKGKTKASQVK RATRG20 310 12
RSERGPRAKSKARASQVK A2AA_PIG 310 12
RPERGLRAKSKARASQVK CPU25722 310 12
ADRENRGCA2AR7 Length of motif = 12 Motif number = 7
A2A adrenergic receptor motif VII - 1
PCODE ST INT
FKKILCRGDRKR A2AA_HUMAN 437 109
FKKILCRGDRKR A2AA_MOUSE 437 109
FKKILCRGDRKR A2AA_RAT 437 109
FKKILCRGDRKR HUMADRA 437 109
FKKILCRGDRKR HUMADRA2R 437 109
FKKILCRGDRKR RATRG20 437 109
FKKILCRGDRKR A2AA_PIG 437 109
FKKILCRGDRKR CPU25722 437 109
FINAL MOTIF SETS
ADRENRGCA2AR1 Length of motif = 23 Motif number = 1
A2A adrenergic receptor motif I - 2
PCODE ST INT
PDAGNSSWNGTEAPGGGTRATPY A2AA_MOUSE 6 6
PDAGNASWNGTEAPGGGARATPY A2AA_HUMAN 6 6
PDAGNSSWNGTEAPGGGTRATPY A2AA_RAT 6 6
PEAGNASWNGTEAPGGGARATPY A2AA_PIG 6 6
PDSGNASWNGTEGPGGGTRATPY A2AA_CAVPO 6 6
ADRENRGCA2AR2 Length of motif = 24 Motif number = 2
A2A adrenergic receptor motif II - 2
PCODE ST INT
SIEKKGAGGGQQPAEPSCKINDQK A2AA_MOUSE 171 142
SIEKKGGGGGPQPAEPRCEINDQK A2AA_HUMAN 171 142
SIEKKGAGGGQQPAEPSCKINDQK A2AA_RAT 171 142
SIEKKAGGGGQQPAEPRCEINDQK A2AA_PIG 171 142
ISFEKAGGGGQQPAEPRCEINDQK A2AA_CAVPO 170 141
ADRENRGCA2AR3 Length of motif = 20 Motif number = 3
A2A adrenergic receptor motif III - 2
PCODE ST INT
PSRRGPDACSAPPGGADRRP A2AA_MOUSE 231 36
PSRRGPDAVAAPPGGTERRP A2AA_HUMAN 231 36
PSRRGPDACSAPPGGADRRP A2AA_RAT 231 36
PSRRGPDAAAALPGGAERRP A2AA_PIG 231 36
PSRRGPDAHAAAPPGGAERR A2AA_CAVPO 230 36
ADRENRGCA2AR4 Length of motif = 20 Motif number = 4
A2A adrenergic receptor motif IV - 2
PCODE ST INT
ERGAGPTGAEAEPLPTQLNG A2AA_MOUSE 256 5
ERSAGPGGAEAEPLPTQLNG A2AA_HUMAN 256 5
ERGAGTAGAEAEPLPTQLNG A2AA_RAT 256 5
ERGVGRVGAEAEPLPVQLNG A2AA_PIG 256 5
ERGVGPGGAEAEPLPTQVNG A2AA_CAVPO 256 6
ADRENRGCA2AR5 Length of motif = 19 Motif number = 5
A2A adrenergic receptor motif V - 2
PCODE ST INT
EPAPAGPRDGDALDLEESS A2AA_MOUSE 279 3
EPAPAGPRDTDALDLEESS A2AA_HUMAN 279 3
EPAPTRPRDGDALDLEESS A2AA_RAT 279 3
EPAPAGPRDADGLDLEESS A2AA_PIG 279 3
EPAPAGPRDAEALDLEESS A2AA_CAVPO 279 3
ADRENRGCA2AR6 Length of motif = 18 Motif number = 6
A2A adrenergic receptor motif VI - 2
PCODE ST INT
RPDRGPRAKGKTRASQVK A2AA_MOUSE 310 12
RPERGPRGKGKARASQVK A2AA_HUMAN 310 12
KPERGPRAKGKTKASQVK A2AA_RAT 310 12
RSERGPRAKSKARASQVK A2AA_PIG 310 12
RPERGLRAKSKARASQVK A2AA_CAVPO 310 12
ADRENRGCA2AR7 Length of motif = 12 Motif number = 7
A2A adrenergic receptor motif VII - 2
PCODE ST INT
FKKILCRGDRKR A2AA_MOUSE 437 109
FKKILCRGDRKR A2AA_HUMAN 437 109
FKKILCRGDRKR A2AA_RAT 437 109
FKKILCRGDRKR A2AA_PIG 437 109
FKKILCRGDRKR A2AA_CAVPO 437 109
User query: Display/Full Code "ADRENRGCA2AR"